2ggh
From Proteopedia
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|PDB= 2ggh |SIZE=350|CAPTION= <scene name='initialview01'>2ggh</scene>, resolution 2.20Å | |PDB= 2ggh |SIZE=350|CAPTION= <scene name='initialview01'>2ggh</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NLQ:N~2~-ACETYL-L-GLUTAMINE'>NLQ</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Amino-acid_racemase Amino-acid racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.10 5.1.1.10] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Amino-acid_racemase Amino-acid racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.10 5.1.1.10] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1r0m|1R0M]], [[2fkp|2FKP]], [[2ggg|2GGG]], [[2ggi|2GGI]], [[2ggj|2GGJ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ggh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ggh OCA], [http://www.ebi.ac.uk/pdbsum/2ggh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ggh RCSB]</span> | ||
}} | }} | ||
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[[Category: Chiu, W C.]] | [[Category: Chiu, W C.]] | ||
[[Category: Wang, W C.]] | [[Category: Wang, W C.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: NLQ]] | ||
[[Category: deinococcus radioduran]] | [[Category: deinococcus radioduran]] | ||
[[Category: n-acylamino acid racemase]] | [[Category: n-acylamino acid racemase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:16:18 2008'' |
Revision as of 00:16, 31 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Amino-acid racemase, with EC number 5.1.1.10 | ||||||
| Related: | 1R0M, 2FKP, 2GGG, 2GGI, 2GGJ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The mutant A68C-D72C-NLQ of Deinococcus Radiodurans Nacylamino acid racemase
Overview
N-Acylamino acid racemase (NAAAR) and N-carbamoyl-D-amino-acid amidohydrolase (D-NCAase) are important biocatalysts for producing enantiopure alpha-amino acids. NAAAR forms an octameric assembly and displays induced fit movements upon substrate binding, while D-NCAase is a tetramer that does not change conformation in the presence of a ligand. To investigate the effects of introducing potentially stabilizing S-S bridges in these different multimeric enzymes, cysteine residues predicted to form inter or intra-subunit disulfide bonds were introduced by site-directed mutagenesis. Inter-subunit S-S bonds were formed in two NAAAR variants (A68C-D72C and P60C-Y100C) and two d-NCAase variants (A302C and P295C-F304C). Intra-subunit S-S bonds were formed in two additional NAAAR variants (E149C-A182C and V265C). Crystal structures of NAAARs variants show limited deviations from the wild-type overall tertiary structure. An apo A68C-D72C subunit differs from the wild-type enzyme, in which it has an ordered lid loop, resembling ligand-bound NAAAR. The structures of A222C and A302C D-NCAases are nearly identical to the wild-type enzyme. All mutants with inter-subunit bridges had increases in thermostability. Compared with the wild-type enzyme, A68C-D72C NAAAR showed similar kcat/Km ratios, whereas mutant D-NCAases demonstrated increased kcat/Km ratios at high temperatures (A302C: 4.2-fold at 65 degrees C). Furthermore, molecular dynamic simulations reveal that A302C substantially sustains the fine-tuned catalytic site as temperature increases, achieving enhanced activity.
About this Structure
2GGH is a Single protein structure of sequence from Deinococcus radiodurans. This structure supersedes the now removed PDB entries 2FKR and 2BA8. Full crystallographic information is available from OCA.
Reference
Structure-stability-activity relationship in covalently cross-linked N-carbamoyl D-amino acid amidohydrolase and N-acylamino acid racemase., Chiu WC, You JY, Liu JS, Hsu SK, Hsu WH, Shih CH, Hwang JK, Wang WC, J Mol Biol. 2006 Jun 9;359(3):741-53. Epub 2006 Apr 18. PMID:16650857
Page seeded by OCA on Mon Mar 31 03:16:18 2008
