2gim
From Proteopedia
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|PDB= 2gim |SIZE=350|CAPTION= <scene name='initialview01'>2gim</scene>, resolution 1.60Å | |PDB= 2gim |SIZE=350|CAPTION= <scene name='initialview01'>2gim</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1172 Anabaena variabilis]) | |GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1172 Anabaena variabilis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gim OCA], [http://www.ebi.ac.uk/pdbsum/2gim PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gim RCSB]</span> | ||
}} | }} | ||
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[[Category: Harris, P.]] | [[Category: Harris, P.]] | ||
[[Category: Schmidt, L.]] | [[Category: Schmidt, L.]] | ||
| - | [[Category: CU]] | ||
[[Category: beta sheet]] | [[Category: beta sheet]] | ||
[[Category: cu]] | [[Category: cu]] | ||
[[Category: helix]] | [[Category: helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:17:07 2008'' |
Revision as of 00:17, 31 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | petE (Anabaena variabilis) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.6 Angstrom structure of plastocyanin from Anabaena variabilis
Overview
Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.
About this Structure
2GIM is a Single protein structure of sequence from Anabaena variabilis. Full crystallographic information is available from OCA.
Reference
Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103
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