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2gtj

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|ACTIVITY=
|ACTIVITY=
|GENE= FYB, SLAP130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
|GENE= FYB, SLAP130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gtj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gtj OCA], [http://www.ebi.ac.uk/pdbsum/2gtj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gtj RCSB]</span>
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[[Category: sh3]]
[[Category: sh3]]
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Revision as of 00:21, 31 March 2008

Image:2gtj.jpg


PDB ID 2gtj

Drag the structure with the mouse to rotate
Gene: FYB, SLAP130 (Homo sapiens)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Reduced form of ADAP hSH3-N-domain


Overview

Oxidation-induced conformational changes in proteins provide a powerful mechanism to sense the redox state of a living cell. In contrast to the unspecific and often irreversible oxidation of intracellular proteins during severe oxidative stress, regulatory redox events need to have specific and transient effects on cellular targets. Here we present evidence for the reversible formation of a vicinal disulfide bond in a prototypic protein interaction domain. NMR spectroscopy was used to determine the structure of the N-terminal hSH3 domain (hSH3N) of the immune cell protein ADAP (adhesion and degranulation promoting adapter protein) in the reduced and oxidized states. An eight-membered ring formed upon oxidation of two neighboring cysteines leads to significant changes in the variable arginine-threonine (RT) loop of the hSH3N domain and alters the helix-sheet packing of the domain. The redox potential for this structural transition is -228 mV at pH 7.4. This is compatible with a role of the cysteinylcysteine moiety in redox signaling during T cell activation.

About this Structure

2GTJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Redox-regulated conformational changes in an SH3 domain., Zimmermann J, Kuhne R, Sylvester M, Freund C, Biochemistry. 2007 Jun 12;46(23):6971-7. Epub 2007 May 19. PMID:17511475

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