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4bwq

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Revision as of 18:58, 11 August 2016

Crystal structure of U5-15kD in a complex with PQBP1

Structural highlights

4bwq is a 8 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4bws
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[PQBP1_HUMAN] X-linked intellectual deficit, Sutherland-Haan type;X-linked intellectual deficit, Golabi-Ito-Hall type;X-linked intellectual deficit, Porteous type;Hamel cerebro-palato-cardiac syndrome. The disease is caused by mutations affecting the gene represented in this entry.

Function

[TXN4A_HUMAN] Essential role in pre-mRNA splicing. [PQBP1_HUMAN] May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit.^[1] ^[2] ^[3]

Publication Abstract from PubMed

A loss-of-function of polyglutamine tract-binding protein 1 (PQBP1) induced by frameshift mutations is believed to cause X-linked mental retardation. However, the mechanism by which structural changes in PQBP1 lead to mental retardation is unknown. Here we present the crystal structure of a C-terminal fragment of PQBP1 in complex with the spliceosomal protein U5-15kD. The U5-15kD hydrophobic groove recognizes a YxxPxxVL motif in PQBP1, and mutations within this motif cause a loss-of-function phenotype of PQBP1 in vitro. The YxxPxxVL motif is absent in all PQBP1 frameshift mutants seen in cases of mental retardation. These results suggest a mechanism by which the loss of the YxxPxxVL motif could lead to the functional defects seen in this type of mental retardation.

Mutations in the PQBP1 gene prevent its interaction with the spliceosomal protein U5-15kD.,Mizuguchi M, Obita T, Serita T, Kojima R, Nabeshima Y, Okazawa H Nat Commun. 2014 Apr 30;5:3822. doi: 10.1038/ncomms4822. PMID:24781215^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Waragai M, Lammers CH, Takeuchi S, Imafuku I, Udagawa Y, Kanazawa I, Kawabata M, Mouradian MM, Okazawa H. PQBP-1, a novel polyglutamine tract-binding protein, inhibits transcription activation by Brn-2 and affects cell survival. Hum Mol Genet. 1999 Jun;8(6):977-87. PMID:10332029
↑ Komuro A, Saeki M, Kato S. Npw38, a novel nuclear protein possessing a WW domain capable of activating basal transcription. Nucleic Acids Res. 1999 May 1;27(9):1957-65. PMID:10198427
↑ Okazawa H, Rich T, Chang A, Lin X, Waragai M, Kajikawa M, Enokido Y, Komuro A, Kato S, Shibata M, Hatanaka H, Mouradian MM, Sudol M, Kanazawa I. Interaction between mutant ataxin-1 and PQBP-1 affects transcription and cell death. Neuron. 2002 May 30;34(5):701-13. PMID:12062018
↑ Mizuguchi M, Obita T, Serita T, Kojima R, Nabeshima Y, Okazawa H. Mutations in the PQBP1 gene prevent its interaction with the spliceosomal protein U5-15kD. Nat Commun. 2014 Apr 30;5:3822. doi: 10.1038/ncomms4822. PMID:24781215 doi:http://dx.doi.org/10.1038/ncomms4822

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4bwq"

@@ Line 1: / Line 1: @@
 ==Crystal structure of U5-15kD in a complex with PQBP1==
 <StructureSection load='4bwq' size='340' side='right' caption='[[4bwq]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4bwq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BWQ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bws|4bws]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bwq RCSB], [http://www.ebi.ac.uk/pdbsum/4bwq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwq OCA], [http://pdbe.org/4bwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bwq RCSB], [http://www.ebi.ac.uk/pdbsum/4bwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwq ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4bwq" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4bwq

From Proteopedia

Revision as of 18:58, 11 August 2016

Crystal structure of U5-15kD in a complex with PQBP1

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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