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2h6u

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|ACTIVITY=
|ACTIVITY=
|GENE= GENEID:558664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Danio rerio])
|GENE= GENEID:558664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Danio rerio])
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|DOMAIN=
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|RELATEDENTRY=[[2h1x|2H1X]], [[1f41|1F41]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h6u OCA], [http://www.ebi.ac.uk/pdbsum/2h6u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h6u RCSB]</span>
}}
}}
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[[Category: uric acid degradation]]
[[Category: uric acid degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:13:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:16 2008''

Revision as of 00:26, 31 March 2008


PDB ID 2h6u

Drag the structure with the mouse to rotate
, resolution 1.70Å
Gene: GENEID:558664 (Danio rerio)
Related: 2H1X, 1F41


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of 5-hydroxyisourate hydrolase (formerly known as TRP, transthyretin related protein)


Overview

During early vertebrate evolution, a duplication event in the gene encoding 5-hydroxyisourate hydrolase (HIUase), a widely distributed enzyme of purine metabolism, gave rise to transthyretin (TTR), a thyroid hormone transporter. We report here on the crystal structure of zebra fish HIUase in two different crystal forms. Despite the phylogenetic distance, this structure compares well with those of newly characterized bacterial HIUases, especially with regard to catalytic regions, which are highly preserved. Comparison with TTR structure reveals a highly conserved scaffold, harbouring distinct functional sites located in the same regions of the two vertebrate proteins. Residues that are differentially conserved in HIUases compared to TTR map in putative catalytic regions occupying significant portions of the two halves of a central channel that transverses the whole TTR protein. The evolution of TTR has been accompanied by remarkable changes of the HIUase active sites that gave rise to a channel open at both ends, thus allowing free access to hormone molecules.

About this Structure

2H6U is a Single protein structure of sequence from Danio rerio. Full crystallographic information is available from OCA.

Reference

Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter., Zanotti G, Cendron L, Ramazzina I, Folli C, Percudani R, Berni R, J Mol Biol. 2006 Oct 13;363(1):1-9. Epub 2006 Aug 2. PMID:16952372

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