2h9x

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|PDB= 2h9x |SIZE=350|CAPTION= <scene name='initialview01'>2h9x</scene>
|PDB= 2h9x |SIZE=350|CAPTION= <scene name='initialview01'>2h9x</scene>
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|LIGAND= <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9x OCA], [http://www.ebi.ac.uk/pdbsum/2h9x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h9x RCSB]</span>
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[[Category: beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:27:28 2008''

Revision as of 00:27, 31 March 2008


PDB ID 2h9x

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Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



NMR structure for the CgNa toxin from the sea anemone Condylactis gigantea


Overview

CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to the canonical structural elements that are thought to be essential for the activity of this group of toxins. Furthermore, unique residues in CgNa define a characteristic structure with strong negatively charged surface patches. These patches disrupt a surface-exposed cluster of hydrophobic residues present in all anemone-derived toxins described to date. A thorough characterization by patch-clamp analysis using rat DRG (dorsal root ganglion) neurons indicated that CgNa preferentially binds to TTX-S (tetrodotoxin-sensitive) voltage-gated sodium channels in the resting state. This association increased the inactivation time constant and the rate of recovery from inactivation, inducing a significant shift in the steady state of inactivation curve to the left. The specific structural features of CgNa may explain its weaker inhibitory capacity when compared with the other type I and II anemone toxins.

About this Structure

2H9X is a Single protein structure of sequence from Condylactis gigantea. Full crystallographic information is available from OCA.

Reference

CgNa, a type I toxin from the giant Caribbean sea anemone Condylactis gigantea shows structural similarities to both type I and II toxins, as well as distinctive structural and functional properties(1)., Salceda E, Perez-Castells J, Lopez-Mendez B, Garateix A, Salazar H, Lopez O, Aneiros A, Standker L, Beress L, Forssmann WG, Soto E, Jimenez-Barbero J, Gimenez-Gallego G, Biochem J. 2007 Aug 15;406(1):67-76. PMID:17506725

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