2hdi
From Proteopedia
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|PDB= 2hdi |SIZE=350|CAPTION= <scene name='initialview01'>2hdi</scene>, resolution 2.50Å | |PDB= 2hdi |SIZE=350|CAPTION= <scene name='initialview01'>2hdi</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=LDA:LAURYL DIMETHYLAMINE-N-OXIDE'>LDA</scene> | + | |LIGAND= <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= cirA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), cia ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= cirA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), cia ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2hdf|2HDF]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hdi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hdi OCA], [http://www.ebi.ac.uk/pdbsum/2hdi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hdi RCSB]</span> | ||
}} | }} | ||
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[[Category: Esser, L.]] | [[Category: Esser, L.]] | ||
[[Category: Lukacik, P.]] | [[Category: Lukacik, P.]] | ||
| - | [[Category: LDA]] | ||
[[Category: colicin i receptor]] | [[Category: colicin i receptor]] | ||
[[Category: iron transport]] | [[Category: iron transport]] | ||
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[[Category: tonb box]] | [[Category: tonb box]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:28:50 2008'' |
Revision as of 00:28, 31 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | cirA (Escherichia coli), cia (Escherichia coli) | ||||||
| Related: | 2HDF
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Colicin I receptor Cir from E.coli in complex with receptor binding domain of Colicin Ia.
Overview
Colicin Ia is a 69 kDa protein that kills susceptible Escherichia coli cells by binding to a specific receptor in the outer membrane, colicin I receptor (70 kDa), and subsequently translocating its channel forming domain across the periplasmic space, where it inserts into the inner membrane and forms a voltage-dependent ion channel. We determined crystal structures of colicin I receptor alone and in complex with the receptor binding domain of colicin Ia. The receptor undergoes large and unusual conformational changes upon colicin binding, opening at the cell surface and positioning the receptor binding domain of colicin Ia directly above it. We modelled the interaction with full-length colicin Ia to show that the channel forming domain is initially positioned 150 A above the cell surface. Functional data using full-length colicin Ia show that colicin I receptor is necessary for cell surface binding, and suggest that the receptor participates in translocation of colicin Ia across the outer membrane.
About this Structure
2HDI is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import., Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L, EMBO J. 2007 May 16;26(10):2594-604. Epub 2007 Apr 26. PMID:17464289
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