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2het
From Proteopedia
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|PDB= 2het |SIZE=350|CAPTION= <scene name='initialview01'>2het</scene>, resolution 3.0Å | |PDB= 2het |SIZE=350|CAPTION= <scene name='initialview01'>2het</scene>, resolution 3.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= RCV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]) | |GENE= RCV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2het FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2het OCA], [http://www.ebi.ac.uk/pdbsum/2het PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2het RCSB]</span> | ||
}} | }} | ||
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[[Category: Granzin, J.]] | [[Category: Granzin, J.]] | ||
[[Category: Weiergraber, O H.]] | [[Category: Weiergraber, O H.]] | ||
| - | [[Category: CA]] | ||
[[Category: calcium binding]] | [[Category: calcium binding]] | ||
[[Category: ef-hand]] | [[Category: ef-hand]] | ||
| Line 30: | Line 32: | ||
[[Category: recoverin]] | [[Category: recoverin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:29:21 2008'' |
Revision as of 00:29, 31 March 2008
| |||||||
| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | RCV1 (Bos taurus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3
Overview
Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in the vertebrate retina that has been implicated in visual adaptation. An intriguing feature of recoverin is a cluster of charged residues at its C terminus, the functional significance of which is largely unclear. To elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids. Whereas in myristoylated recoverin the truncation causes an overall decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant indicate that the truncation primarily affects the high affinity EF-hand 3. The three-dimensional structure of the mutant has been determined by x-ray crystallography. In addition to significant changes in average coordinates compared with wild-type recoverin, the structure provides strong indication of increased conformational flexibility, particularly in the C-terminal domain. Based on these observations, we propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity.
About this Structure
2HET is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Tuning of a neuronal calcium sensor., Weiergraber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2006 Dec 8;281(49):37594-602. Epub 2006 Oct 2. PMID:17015448
Page seeded by OCA on Mon Mar 31 03:29:21 2008
