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3uxi

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Revision as of 14:13, 11 August 2016

Crystal structure of L-rhamnose isomerase W38A mutant from Bacillus halodurans

Structural highlights

3uxi is a 4 chain structure with sequence from "bacillus_alcalophilus_subsp._halodurans"_boyer_et_al._1973 "bacillus alcalophilus subsp. halodurans" boyer et al. 1973. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3p14, 3uu0, 3uva
Gene:	rhaA, BH1552 ("Bacillus alcalophilus subsp. halodurans" Boyer et al. 1973)
Activity:	L-rhamnose isomerase, with EC number 5.3.1.14
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Two-metal-dependent sugar isomerases are important in the synthesis of rare sugars. Many of their properties, specifically their metal dependency, have not been sufficiently explored. Here we used X-ray crystallography, site-directed mutagenesis, isothermal titration calorimetry and electron paramagnetic resonance spectroscopy to investigate the molecular determinants of the metal-binding affinity of l-rhamnose isomerase, a two-Mn(2+) -dependent isomerase from Bacillus halodurans (BHRI). The crystal structure of BHRI confirmed the presence of two metal ion-binding sites: a structural metal ion-binding site for substrate binding, and a catalytic metal ion-binding site that catalyzes a hydride shift. One conserved amino acid, W38, in wild-type BHRI was identified as a critical residue for structural Mn(2+) binding and thus the catalytic efficiency of BHRI. This function of W38 was explored by replacing it with other amino acids. Substitution by Phe, His, Lys, Ile or Ala caused complete loss of catalytic activity. The role of W38 was further examined by analyzing the crystal structure of wild-type BHRI and two inactive mutants of BHRI (W38F and W38A) in complex with Mn(2+) . A structural comparison of the mutants and the wild-type revealed differences in their coordination of Mn(2+) , including changes in metal-ligand bond length and affinity for Mn(2+) . The role of W38 was further confirmed in another two-metal-dependent enzyme: xylose isomerase from Bacillus licheniformis. These data suggest that W38 stabilizes protein-metal complexes and in turn assists ligand binding during catalysis in two-metal-dependent isomerases. STRUCTURED DIGITAL ABSTRACT: BHRI and BHRI bind by x-ray crystallography (View interaction).

Structure-based studies on the metal binding of two-metal-dependent sugar isomerases.,Prabhu P, Doan TN, Tiwari M, Singh R, Kim SC, Hong MK, Kang YC, Kang LW, Lee JK FEBS J. 2014 Aug;281(15):3446-59. doi: 10.1111/febs.12872. Epub 2014 Jul 4. PMID:24925069^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Prabhu P, Doan TN, Tiwari M, Singh R, Kim SC, Hong MK, Kang YC, Kang LW, Lee JK. Structure-based studies on the metal binding of two-metal-dependent sugar isomerases. FEBS J. 2014 Aug;281(15):3446-59. doi: 10.1111/febs.12872. Epub 2014 Jul 4. PMID:24925069 doi:http://dx.doi.org/10.1111/febs.12872

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3uxi"

@@ Line 1: / Line 1: @@
 ==Crystal structure of L-rhamnose isomerase W38A mutant from Bacillus halodurans==
 <StructureSection load='3uxi' size='340' side='right' caption='[[3uxi]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rhaA, BH1552 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86665 "Bacillus alcalophilus subsp. halodurans" Boyer et al. 1973])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-rhamnose_isomerase L-rhamnose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.14 5.3.1.14] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uxi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uxi RCSB], [http://www.ebi.ac.uk/pdbsum/3uxi PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uxi OCA], [http://pdbe.org/3uxi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uxi RCSB], [http://www.ebi.ac.uk/pdbsum/3uxi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uxi ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3uxi" style="background-color:#fffaf0;"></div>
 ==See Also==

3uxi

From Proteopedia

Revision as of 14:13, 11 August 2016

Crystal structure of L-rhamnose isomerase W38A mutant from Bacillus halodurans

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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