2wcm

From Proteopedia

(Difference between revisions)

Revision as of 18:06, 11 August 2016

STRUCTURE OF BMORI GOBP2 (GENERAL ODORANT BINDING PROTEIN 2) WITH (10E)-HEXADECEN-12-YN-1-OL

Structural highlights

2wcm is a 1 chain structure with sequence from Bommo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2wc6, 2wcl, 2wch, 2wck, 2wc5, 2wcj
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[OBP1_BOMMO] Present in the aqueous fluid surrounding olfactory sensory dendrites and are thought to aid in the capture and transport of hydrophobic odorants into and through this fluid.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In many insect species, odorant-binding proteins (OBPs) are thought to be responsible for the transport of pheromones and other semiochemicals across the sensillum lymph to the olfactory receptors (ORs) within the antennal sensilla. In the silkworm Bombyx mori, the OBPs are subdivided into three main subfamilies; pheromone-binding proteins (PBPs), general odorant-binding proteins (GOBPs) and antennal-binding proteins (ABPs). We used the MotifSearch algorithm to search for genes encoding putative OBPs in B. mori and found 13, many fewer than are found in the genomes of fruit flies and mosquitoes. The 13 genes include seven new ABP-like OBPs as well as the previously identified PBPs (three), GOBPs (two) and ABPx. Quantitative examination of transcript levels showed that BmorPBP1, BmorGOBP1, BmorGOBP2 and BmorABPx are expressed at very high levels in the antennae and so could be involved in olfaction. A new two-phase binding assay, along with other established assays, showed that BmorPBP1, BmorPBP2, BmorGOBP2 and BmorABPx all bind to the B. mori sex pheromone component (10E,12Z)-hexadecadien-1-ol (bombykol). BmorPBP1, BmorPBP2 and BmorABPx also bind the pheromone component (10E,12Z)-hexadecadienal (bombykal) equally well, whereas BmorGOBP2 can discriminate between bombykol and bombykal. X-ray structures show that when bombykol is bound to BmorGOBP2 it adopts a different conformation from that found when it binds to BmorPBP1. Binding to BmorGOBP2 involves hydrogen bonding to Arg110 rather than to Ser56 as found for BmorPBP1.

Characterisation of Bombyx mori Odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components.,Zhou JJ, Robertson G, He X, Dufour S, Hooper AM, Pickett JA, Keep NH, Field LM J Mol Biol. 2009 Jun 12;389(3):529-45. Epub 2009 Apr 14. PMID:19371749^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou JJ, Robertson G, He X, Dufour S, Hooper AM, Pickett JA, Keep NH, Field LM. Characterisation of Bombyx mori Odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components. J Mol Biol. 2009 Jun 12;389(3):529-45. Epub 2009 Apr 14. PMID:19371749 doi:10.1016/j.jmb.2009.04.015

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wcm"

@@ Line 1: / Line 1: @@
 ==STRUCTURE OF BMORI GOBP2 (GENERAL ODORANT BINDING PROTEIN 2) WITH (10E)-HEXADECEN-12-YN-1-OL==
 <StructureSection load='2wcm' size='340' side='right' caption='[[2wcm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wcm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WCM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WCM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wcm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WCM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WCM FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B9M:(10E)-HEXADEC-10-EN-12-YN-1-OL'>B9M</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wc6|2wc6]], [[2wcl|2wcl]], [[2wch|2wch]], [[2wck|2wck]], [[2wc5|2wc5]], [[2wcj|2wcj]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wcm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wcm RCSB], [http://www.ebi.ac.uk/pdbsum/2wcm PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wcm OCA], [http://pdbe.org/2wcm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wcm RCSB], [http://www.ebi.ac.uk/pdbsum/2wcm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wcm ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wcm ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 27: / Line 28: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2wcm" style="background-color:#fffaf0;"></div>
 ==See Also==
+*[[Odorant binding protein|Odorant binding protein]]
 *[[Odorant binding protein 3D structures|Odorant binding protein 3D structures]]
 == References ==
@@ Line 34: / Line 37: @@
 __TOC__
 </StructureSection>
-[[Category: Bombyx mori]]
+[[Category: Bommo]]
 [[Category: Field, L M]]
 [[Category: He, X]]

2wcm

From Proteopedia

Revision as of 18:06, 11 August 2016

STRUCTURE OF BMORI GOBP2 (GENERAL ODORANT BINDING PROTEIN 2) WITH (10E)-HEXADECEN-12-YN-1-OL

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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