Sandbox Reserved 951

From Proteopedia

proteopedia linkproteopedia link

Revision as of 22:50, 7 January 2015 (edit) Amandine Schmit (Talk | contribs) ← Previous diff		Revision as of 23:15, 7 January 2015 (edit) (undo) Amandine Schmit (Talk | contribs) Next diff →
Line 9:		Line 9:
	== Function highlighted with structure ==		== Function highlighted with structure ==
-	The most known reaction of luciferase is the light emission.	+	=== The different reactions===
		+
		+	====Light emission====
		+	The most known reaction of luciferase is the light emission. In this reaction, luciferase firstly synthetized luciferin-AMP from luciferin and ATP, using a Mg2+ ion to offset the negative charges of the phosphate groups. Then, luciferase turns the luciferin-AMP into oxyluciferin in an excited state thanks to a dioxygen. This step releases AMP and CO2. The excited oxyluciferin relaxes and looses a photon so light is emitted.
		+	The wavelength of the light can vary with the pH : at the physiological pH, the emitted light is green and at a lower pH, the color is red.
		+
		+	====Fatty-acyl-CoA synthesis====
		+	This reaction uses the luciferin-AMP which is created at the first step of light emission. But then, CoA-SH attacks the oxygen of the carboxylic group to form fatty-acyl-CoA and releases AMP.
	== Evolution ==		== Evolution ==

---

Revision as of 23:15, 7 January 2015

spinqualitylabelsall models Crystal structure of firefly luciferase Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Contents 1 Introduction 2 Biological role 3 Global Structure 4 Function highlighted with structure 4.1 The different reactions 4.1.1 Light emission 4.1.2 Fatty-acyl-CoA synthesis 5 Evolution 6 References

Introduction

Biological role

Global Structure

Function highlighted with structure

The different reactions

Light emission

The most known reaction of luciferase is the light emission. In this reaction, luciferase firstly synthetized luciferin-AMP from luciferin and ATP, using a Mg2+ ion to offset the negative charges of the phosphate groups. Then, luciferase turns the luciferin-AMP into oxyluciferin in an excited state thanks to a dioxygen. This step releases AMP and CO2. The excited oxyluciferin relaxes and looses a photon so light is emitted. The wavelength of the light can vary with the pH : at the physiological pH, the emitted light is green and at a lower pH, the color is red.

Fatty-acyl-CoA synthesis

This reaction uses the luciferin-AMP which is created at the first step of light emission. But then, CoA-SH attacks the oxygen of the carboxylic group to form fatty-acyl-CoA and releases AMP.

Evolution

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>

References