We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.
4x8b
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==Ergothioneine-biosynthetic sulfoxide synthase EgtB, apo form== |
| + | <StructureSection load='4x8b' size='340' side='right' caption='[[4x8b]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4x8b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X8B FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x8b RCSB], [http://www.ebi.ac.uk/pdbsum/4x8b PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The non-heme iron enzyme EgtB catalyzes O2 -dependent CS bond formation between gamma-glutamyl cysteine and N-alpha-trimethyl histidine as the central step in ergothioneine biosynthesis. Both, the catalytic activity and the architecture of EgtB are distinct from known sulfur transferases or thiol dioxygenases. The crystal structure of EgtB from Mycobacterium thermoresistibile in complex with gamma-glutamyl cysteine and N-alpha-trimethyl histidine reveals that the two substrates and three histidine residues serve as ligands in an octahedral iron binding site. This active site geometry is consistent with a catalytic mechanism in which CS bond formation is initiated by an iron(III)-complexed thiyl radical attacking the imidazole ring of N-alpha-trimethyl histidine. | ||
| - | + | Structure of the Sulfoxide Synthase EgtB from the Ergothioneine Biosynthetic Pathway.,Goncharenko KV, Vit A, Blankenfeldt W, Seebeck FP Angew Chem Int Ed Engl. 2015 Jan 16. doi: 10.1002/anie.201410045. PMID:25597398<ref>PMID:25597398</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Blankenfeldt, W]] | [[Category: Blankenfeldt, W]] | ||
| - | [[Category: Goncharenko, K | + | [[Category: Goncharenko, K V]] |
| + | [[Category: Seebeck, F P]] | ||
[[Category: Vit, A]] | [[Category: Vit, A]] | ||
| + | [[Category: C-lectin]] | ||
| + | [[Category: Dinb]] | ||
| + | [[Category: Ergothioneine biosynthesis]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 16:39, 28 January 2015
Ergothioneine-biosynthetic sulfoxide synthase EgtB, apo form
| |||||||||||
