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4ulw

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Revision as of 15:05, 11 August 2016

Crystal structure of the ROQ-domain of human ROQUIN1

Structural highlights

4ulw is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RC3H1_HUMAN] Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs (By similarity). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity (By similarity). In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression (By similarity). Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation (By similarity).

Publication Abstract from PubMed

Roquin proteins mediate mRNA deadenylation by recognizing a conserved class of stem-loop RNA degradation motifs via their Roquin domain. Here we present the crystal structure of a Roquin domain, revealing a mostly helical protein fold bearing a winged helix-turn-helix motif. By combining structural, biochemical and mutation analyses, we gain insight into the mode of RNA binding. We show that the winged helix-turn-helix motif is involved in the binding of constitutive decay elements-containing stem-loop mRNAs. Moreover, we provide biochemical evidence that Roquin proteins are additionally able to bind to duplex RNA and have the potential to be functional in different oligomeric states.

Roquin binding to target mRNAs involves a winged helix-turn-helix motif.,Schuetz A, Murakawa Y, Rosenbaum E, Landthaler M, Heinemann U Nat Commun. 2014 Dec 11;5:5701. doi: 10.1038/ncomms6701. PMID:25504471^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schuetz A, Murakawa Y, Rosenbaum E, Landthaler M, Heinemann U. Roquin binding to target mRNAs involves a winged helix-turn-helix motif. Nat Commun. 2014 Dec 11;5:5701. doi: 10.1038/ncomms6701. PMID:25504471 doi:http://dx.doi.org/10.1038/ncomms6701

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ulw"

Categories: Heinemann, U | Schuetz, A | Rna-binding protein

@@ Line 1: / Line 1: @@
 ==Crystal structure of the ROQ-domain of human ROQUIN1==
 <StructureSection load='4ulw' size='340' side='right' caption='[[4ulw]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4ulw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ULW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ULW FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ulw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ulw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ulw RCSB], [http://www.ebi.ac.uk/pdbsum/4ulw PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ulw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ulw OCA], [http://pdbe.org/4ulw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ulw RCSB], [http://www.ebi.ac.uk/pdbsum/4ulw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ulw ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4ulw" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4ulw

From Proteopedia

Revision as of 15:05, 11 August 2016

Crystal structure of the ROQ-domain of human ROQUIN1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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