2jeu
From Proteopedia
Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
+ | |DOMAIN= | ||
+ | |RELATEDENTRY= | ||
+ | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jeu OCA], [http://www.ebi.ac.uk/pdbsum/2jeu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jeu RCSB]</span> | ||
}} | }} | ||
Line 44: | Line 47: | ||
[[Category: viral transcription factor]] | [[Category: viral transcription factor]] | ||
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:57:12 2008'' |
Revision as of 00:57, 31 March 2008
| |||||||
, resolution 2.80Å | |||||||
---|---|---|---|---|---|---|---|
Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
Coordinates: | save as pdb, mmCIF, xml |
TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION
Overview
Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.
About this Structure
2JEU is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495
Page seeded by OCA on Mon Mar 31 03:57:12 2008
Categories: Bovine papillomavirus type 1 | Single protein | Antson, A A. | Ortiz-Lombardia, M. | Sanders, C M. | Seavers, P R. | Sizov, D. | Activator | Bovine papillomavirus | Dna replication | Dna-binding | E1 | E2 | Early protein | Nuclear protein | Oxidation | Phosphorylation | Redox control | Replication initiation | Repressor | Transcription | Transcription regulation | Viral transcription factor