2jih
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2jih |SIZE=350|CAPTION= <scene name='initialview01'>2jih</scene>, resolution 2.1Å | |PDB= 2jih |SIZE=350|CAPTION= <scene name='initialview01'>2jih</scene>, resolution 2.1Å | ||
|SITE= <scene name='pdbsite=AC1:097+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:097+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Cd+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Cd+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=AC7:Ni+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Cd+Binding+Site+For+Chain+A'>AC8</scene>, <scene name='pdbsite=AC9:Cd+Binding+Site+For+Chain+A'>AC9</scene>, <scene name='pdbsite=BC1:Cd+Binding+Site+For+Chain+B'>BC1</scene>, <scene name='pdbsite=BC2:Ni+Binding+Site+For+Chain+B'>BC2</scene>, <scene name='pdbsite=BC3:Cd+Binding+Site+For+Chain+B'>BC3</scene>, <scene name='pdbsite=BC4:Ni+Binding+Site+For+Chain+B'>BC4</scene>, <scene name='pdbsite=BC5:Ni+Binding+Site+For+Chain+B'>BC5</scene>, <scene name='pdbsite=BC6:Ni+Binding+Site+For+Chain+A'>BC6</scene>, <scene name='pdbsite=BC7:Mg+Binding+Site+For+Chain+A'>BC7</scene>, <scene name='pdbsite=BC8:Ni+Binding+Site+For+Chain+A'>BC8</scene>, <scene name='pdbsite=BC9:Ni+Binding+Site+For+Chain+A'>BC9</scene>, <scene name='pdbsite=CC1:Ni+Binding+Site+For+Chain+A'>CC1</scene>, <scene name='pdbsite=CC2:Ni+Binding+Site+For+Chain+A'>CC2</scene>, <scene name='pdbsite=CC3:Mg+Binding+Site+For+Chain+B'>CC3</scene>, <scene name='pdbsite=CC4:Ni+Binding+Site+For+Chain+B'>CC4</scene>, <scene name='pdbsite=CC7:Na+Binding+Site+For+Chain+A'>CC7</scene>, <scene name='pdbsite=CC8:Na+Binding+Site+For+Chain+A'>CC8</scene>, <scene name='pdbsite=CC9:Na+Binding+Site+For+Chain+A'>CC9</scene>, <scene name='pdbsite=DC1:Na+Binding+Site+For+Chain+B'>DC1</scene>, <scene name='pdbsite=DC2:Na+Binding+Site+For+Chain+B'>DC2</scene>, <scene name='pdbsite=DC3:Ni+Binding+Site+For+Chain+A'>DC3</scene> and <scene name='pdbsite=DC4:Mg+Binding+Site+For+Chain+A'>DC4</scene> | |SITE= <scene name='pdbsite=AC1:097+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:097+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Zn+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Cd+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Cd+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=AC7:Ni+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Cd+Binding+Site+For+Chain+A'>AC8</scene>, <scene name='pdbsite=AC9:Cd+Binding+Site+For+Chain+A'>AC9</scene>, <scene name='pdbsite=BC1:Cd+Binding+Site+For+Chain+B'>BC1</scene>, <scene name='pdbsite=BC2:Ni+Binding+Site+For+Chain+B'>BC2</scene>, <scene name='pdbsite=BC3:Cd+Binding+Site+For+Chain+B'>BC3</scene>, <scene name='pdbsite=BC4:Ni+Binding+Site+For+Chain+B'>BC4</scene>, <scene name='pdbsite=BC5:Ni+Binding+Site+For+Chain+B'>BC5</scene>, <scene name='pdbsite=BC6:Ni+Binding+Site+For+Chain+A'>BC6</scene>, <scene name='pdbsite=BC7:Mg+Binding+Site+For+Chain+A'>BC7</scene>, <scene name='pdbsite=BC8:Ni+Binding+Site+For+Chain+A'>BC8</scene>, <scene name='pdbsite=BC9:Ni+Binding+Site+For+Chain+A'>BC9</scene>, <scene name='pdbsite=CC1:Ni+Binding+Site+For+Chain+A'>CC1</scene>, <scene name='pdbsite=CC2:Ni+Binding+Site+For+Chain+A'>CC2</scene>, <scene name='pdbsite=CC3:Mg+Binding+Site+For+Chain+B'>CC3</scene>, <scene name='pdbsite=CC4:Ni+Binding+Site+For+Chain+B'>CC4</scene>, <scene name='pdbsite=CC7:Na+Binding+Site+For+Chain+A'>CC7</scene>, <scene name='pdbsite=CC8:Na+Binding+Site+For+Chain+A'>CC8</scene>, <scene name='pdbsite=CC9:Na+Binding+Site+For+Chain+A'>CC9</scene>, <scene name='pdbsite=DC1:Na+Binding+Site+For+Chain+B'>DC1</scene>, <scene name='pdbsite=DC2:Na+Binding+Site+For+Chain+B'>DC2</scene>, <scene name='pdbsite=DC3:Ni+Binding+Site+For+Chain+A'>DC3</scene> and <scene name='pdbsite=DC4:Mg+Binding+Site+For+Chain+A'>DC4</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=097:N4-(2,2-DIMETHYL-1-METHYLCARBAMOYL-PROPYL)-2,N1-DIHYDROXY-3-ISOBUTYL-SUCCINAMIDE'>097</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2v4b|2V4B]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jih OCA], [http://www.ebi.ac.uk/pdbsum/2jih PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jih RCSB]</span> | ||
}} | }} | ||
| Line 33: | Line 36: | ||
[[Category: Pauptit, R A.]] | [[Category: Pauptit, R A.]] | ||
[[Category: Ting, A.]] | [[Category: Ting, A.]] | ||
| - | [[Category: 097]] | ||
| - | [[Category: CD]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: NI]] | ||
| - | [[Category: ZN]] | ||
[[Category: adamts-1]] | [[Category: adamts-1]] | ||
[[Category: cleavage on pair of basic residue]] | [[Category: cleavage on pair of basic residue]] | ||
| Line 54: | Line 51: | ||
[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:58:42 2008'' |
Revision as of 00:58, 31 March 2008
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| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , , , , , , , , , , , , , , , , , and | ||||||
| Ligands: | , , , , , | ||||||
| Related: | 2V4B
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF HUMAN ADAMTS-1 CATALYTIC DOMAIN AND CYSTEINE-RICH DOMAIN (COMPLEX-FORM)
Overview
The ADAMTS (a disintegrin-like and metalloproteinase domain with thrombospondin type I motifs) family of proteases plays a role in pathological conditions including arthritis, cancer, thrombotic thrombocytopenic purpura and the Ehlers-Danlos type VIIC and Weill-Marchesani genetic syndromes. Here, we report the first crystal structures for a member of the ADAMTS family, ADAMTS-1. Originally cloned as an inflammation-associated gene, ADAMTS-1 has been shown to be involved in tissue remodelling, wound healing and angiogenesis. The crystal structures contain catalytic and disintegrin-like domains, both in the inhibitor-free form and in complex with the inhibitor marimastat. The overall fold of the catalytic domain is similar to related zinc metalloproteinases such as matrix metalloproteinases and ADAMs (a disintegrin and metalloproteinases). The active site contains the expected organisation of residues to coordinate zinc but has a much larger S1' selectivity pocket than ADAM33. The structure also unexpectedly reveals a double calcium-binding site. Also surprisingly, the previously named disintegrin-like domain showed no structural homology to the disintegrin domains of other metalloproteinases such as ADAM10 but is instead very similar in structure to the cysteine-rich domains of other metalloproteinases. Thus, this study suggests that the D (for disintegrin-like) in the nomenclature of ADAMTS enzymes is likely to be a misnomer. The ADAMTS-1 cysteine-rich domain stacks against the active site, suggesting a possible regulatory role.
About this Structure
2JIH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structures of human ADAMTS-1 reveal a conserved catalytic domain and a disintegrin-like domain with a fold homologous to cysteine-rich domains., Gerhardt S, Hassall G, Hawtin P, McCall E, Flavell L, Minshull C, Hargreaves D, Ting A, Pauptit RA, Parker AE, Abbott WM, J Mol Biol. 2007 Nov 2;373(4):891-902. Epub 2007 Aug 2. PMID:17897672
Page seeded by OCA on Mon Mar 31 03:58:42 2008
Categories: Homo sapiens | Single protein | Abbott, W M. | Flavell, L. | Gerhardt, S. | Hargreaves, D. | Hassall, G. | Hawtin, P. | Mccall, E. | Minshull, C. | Parker, A E. | Pauptit, R A. | Ting, A. | Adamts-1 | Cleavage on pair of basic residue | Extracellular matrix | Glycoprotein | Heparin-binding | Hydrolase | Metal-binding | Metalloprotease | Metalloproteinase | Metzincin | Polymorphism | Protease | Zinc | Zymogen
