2jnf
From Proteopedia
| Line 6: | Line 6: | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=500 Thermomicrobium roseum]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [http://www.ebi.ac.uk/pdbsum/2jnf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2JNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. |
==Reference== | ==Reference== | ||
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637342 17637342] | The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637342 17637342] | ||
| - | [[Category: Lethocerus indicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Thermomicrobium roseum]] | ||
[[Category: Bullard, B.]] | [[Category: Bullard, B.]] | ||
[[Category: Nicola, G F.De.]] | [[Category: Nicola, G F.De.]] | ||
| Line 31: | Line 34: | ||
[[Category: troponin c]] | [[Category: troponin c]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:59:41 2008'' |
Revision as of 00:59, 31 March 2008
| |||||||
| Gene: | tnC4 (Thermomicrobium roseum) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of fly troponin C, isoform F1
Overview
To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.
About this Structure
2JNF is a Single protein structure of sequence from Thermomicrobium roseum. Full crystallographic information is available from OCA.
Reference
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:17637342
Page seeded by OCA on Mon Mar 31 03:59:41 2008
