1gze
From Proteopedia
(New page: 200px<br /> <applet load="1gze" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gze, resolution 2.70Å" /> '''STRUCTURE OF THE CL...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GZE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]] with HG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZE OCA]]. | + | 1GZE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]] with HG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZE OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: nad]] | [[Category: nad]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:55:38 2007'' |
Revision as of 08:50, 30 October 2007
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STRUCTURE OF THE CLOSTRIDIUM BOTULINUM C3 EXOENZYME (L177C MUTANT)
Overview
We have solved the crystal structures of Clostridium botulinum C3, exoenzyme free and complexed to NAD in the same crystal form, at 2.7 and, 1.95 A, respectively. The asymmetric unit contains four molecules, which, in the free form, share the same conformation. Upon NAD binding, C3, underwent various conformational changes, whose amplitudes were, differentially limited in the four molecules of the crystal unit. A major, rearrangement concerns the loop that contains the functionally important, ARTT motif (ADP-ribosyltransferase toxin turn-turn). The ARTT loop, undergoes an ample swinging motion to adopt a conformation that covers the, nicotinamide moiety of NAD. In particular, Gln-212, which belongs to the, ARTT motif, flips over from a solvent-exposed environment to a buried, conformation ... [(full description)]
About this Structure
1GZE is a [Single protein] structure of sequence from [Clostridium botulinum] with HG as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
NAD binding induces conformational changes in Rho ADP-ribosylating clostridium botulinum C3 exoenzyme., Menetrey J, Flatau G, Stura EA, Charbonnier JB, Gas F, Teulon JM, Le Du MH, Boquet P, Menez A, J Biol Chem. 2002 Aug 23;277(34):30950-7. Epub 2002 May 23. PMID:12029083
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