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2lyo
From Proteopedia
| Line 5: | Line 5: | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene> | |LIGAND= <scene name='pdbligand=CCN:ACETONITRILE'>CCN</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lyo OCA], [http://www.ebi.ac.uk/pdbsum/2lyo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2lyo RCSB]</span> | ||
}} | }} | ||
| Line 30: | Line 33: | ||
[[Category: Wang, Z.]] | [[Category: Wang, Z.]] | ||
[[Category: Zhu, G.]] | [[Category: Zhu, G.]] | ||
| - | [[Category: CCN]] | ||
[[Category: cross-linked]] | [[Category: cross-linked]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| Line 36: | Line 38: | ||
[[Category: lysozyme]] | [[Category: lysozyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:03:43 2008'' |
Revision as of 01:03, 31 March 2008
| |||||||
| , resolution 1.93Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 90% ACETONITRILE-WATER
Overview
Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.
About this Structure
2LYO is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:9659395
Page seeded by OCA on Mon Mar 31 04:03:43 2008
Categories: Gallus gallus | Lysozyme | Single protein | Huang, Q. | Jia, Y. | Qian, M. | Shao, M. | Tang, Y. | Wang, Z. | Zhu, G. | Cross-linked | Hydrolase | Hydrolase(o-glycosyl)
