2nqp
From Proteopedia
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|PDB= 2nqp |SIZE=350|CAPTION= <scene name='initialview01'>2nqp</scene>, resolution 3.50Å | |PDB= 2nqp |SIZE=350|CAPTION= <scene name='initialview01'>2nqp</scene>, resolution 3.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/tRNA-pseudouridine_synthase_I tRNA-pseudouridine synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.12 5.4.99.12] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-pseudouridine_synthase_I tRNA-pseudouridine synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.12 5.4.99.12] </span> |
|GENE= truA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= truA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1dj0|1DJ0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nqp OCA], [http://www.ebi.ac.uk/pdbsum/2nqp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nqp RCSB]</span> | ||
}} | }} | ||
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[[Category: Hur, S.]] | [[Category: Hur, S.]] | ||
[[Category: Stroud, R M.]] | [[Category: Stroud, R M.]] | ||
| - | [[Category: K]] | ||
[[Category: anticodon stem loop]] | [[Category: anticodon stem loop]] | ||
[[Category: multisite specificity]] | [[Category: multisite specificity]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:57 2008'' |
Revision as of 01:06, 31 March 2008
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| , resolution 3.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | truA (Escherichia coli) | ||||||
| Activity: | tRNA-pseudouridine synthase I, with EC number 5.4.99.12 | ||||||
| Related: | 1DJ0
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA
Overview
Translational accuracy and efficiency depend upon modification of uridines in the tRNA anticodon stem loop (ASL) by a highly conserved pseudouridine synthase TruA. TruA specifically modifies uridines at positions 38, 39, and/or 40 of tRNAs with highly divergent sequences and structures through a poorly characterized mechanism that differs from previously studied RNA-modifying enzymes. The molecular basis for the site and substrate "promiscuity" was studied by determining the crystal structures of E. coli TruA in complex with two different leucyl tRNAs in conjunction with functional assays and computer simulation. The structures capture three stages of the TruA*tRNA reaction, revealing the mechanism by which TruA selects the target site. We propose that TruA utilizes the intrinsic flexibility of the ASL for site promiscuity and also to select against intrinsically stable tRNAs to avoid their overstabilization through pseudouridylation, thereby maintaining the balance between the flexibility and stability required for its biological function.
About this Structure
2NQP is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA., Hur S, Stroud RM, Mol Cell. 2007 Apr 27;26(2):189-203. PMID:17466622
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