2o0i
From Proteopedia
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= bca ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= bca ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=355315 Streptococcus agalactiae serogroup Ia]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ywm|1YWM]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o0i OCA], [http://www.ebi.ac.uk/pdbsum/2o0i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o0i RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2O0I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2O0I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_ia Streptococcus agalactiae serogroup ia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0I OCA]. |
==Reference== | ==Reference== | ||
Identification of a glycosaminoglycan binding region of the alpha C protein that mediates entry of group B Streptococci into host cells., Baron MJ, Filman DJ, Prophete GA, Hogle JM, Madoff LC, J Biol Chem. 2007 Apr;282(14):10526-36. Epub 2007 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17259175 17259175] | Identification of a glycosaminoglycan binding region of the alpha C protein that mediates entry of group B Streptococci into host cells., Baron MJ, Filman DJ, Prophete GA, Hogle JM, Madoff LC, J Biol Chem. 2007 Apr;282(14):10526-36. Epub 2007 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17259175 17259175] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Streptococcus agalactiae serogroup | + | [[Category: Streptococcus agalactiae serogroup ia]] |
[[Category: Baron, M J.]] | [[Category: Baron, M J.]] | ||
[[Category: Filman, D J.]] | [[Category: Filman, D J.]] | ||
| Line 32: | Line 35: | ||
[[Category: surface active protein]] | [[Category: surface active protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:10:59 2008'' |
Revision as of 01:11, 31 March 2008
| |||||||
| , resolution 3.100Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | bca (Streptococcus agalactiae serogroup Ia) | ||||||
| Related: | 1YWM
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
crystal structure of the R185A mutant of the N-terminal domain of the Group B Streptococcus Alpha C protein
Overview
Group B Streptococcus (GBS) frequently colonizes the human gastrointestinal and gynecological tracts and less frequently causes deep tissue infections. The transition between colonization and infection depends upon the ability of the organism to cross epithelial barriers. The alpha C protein (ACP) on the surface of GBS contributes to this process. A virulence factor in mouse models of infection, and prototype for a family of Gram-positive bacterial surface proteins, ACP facilitates GBS entry into human cervical epithelial cells and movement across cell layers. ACP binds to host cell surface glycosaminoglycan (GAG). From crystallography, we have identified a cluster of basic residues (BR2) that is a putative GAG binding area in Domain 2, near the junction of the N-terminal domain of ACP and the first of a series of tandem amino acid repeats. D2-R, a protein construct including this region, binds to cells similarly to full-length ACP. We now demonstrate that the predicted charged BR2 residues confer GAG binding; site-directed mutagenesis of these residues (Arg(172), Arg(185), or Lys(196)) eliminates cell-binding activity of construct D2-R. In addition, we have constructed a GBS strain expressing a variant ACP with a charge-neutralizing substitution at residue 185. This strain enters host cells less effectively than does the wild-type strain and similarly to an ACP null mutant strain. The point mutant strain transcytoses similarly to the wild-type strain. These data indicate that GAG-binding activity underlies ACP-mediated cellular entry of GBS. GBS entry into host cells and transcytosis of host cells may occur by distinct mechanisms.
About this Structure
2O0I is a Single protein structure of sequence from Streptococcus agalactiae serogroup ia. Full crystallographic information is available from OCA.
Reference
Identification of a glycosaminoglycan binding region of the alpha C protein that mediates entry of group B Streptococci into host cells., Baron MJ, Filman DJ, Prophete GA, Hogle JM, Madoff LC, J Biol Chem. 2007 Apr;282(14):10526-36. Epub 2007 Jan 26. PMID:17259175
Page seeded by OCA on Mon Mar 31 04:10:59 2008
