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2vxq

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Revision as of 15:03, 7 February 2016

CRYSTAL STRUCTURE OF THE MAJOR GRASS POLLEN ALLERGEN PHL P 2 IN COMPLEX WITH ITS SPECIFIC IGE-FAB

Structural highlights

2vxq is a 3 chain structure with sequence from Common timothy and Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1bmw, 1whp, 1who
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report the three-dimensional structure of the complex between the major respiratory grass pollen allergen Phl p 2 and its specific human IgE-derived Fab. The Phl p 2-specific human IgE Fab has been isolated from a combinatorial library constructed from lymphocytes of a pollen allergic patient. When the variable domains of the IgE Fab were grafted onto human IgG1, the resulting Ab (huMab2) inhibited strongly the binding of allergic patients' IgE to Phl p 2 as well as allergen-induced basophil degranulation. Analysis of the binding of the allergen to the Ab by surface plasmon resonance yielded a very low dissociation constant (K(D) = 1.1 x 10(-10) M), which is similar to that between IgE and Fcepsilon;RI. The structure of the Phl p 2/IgE Fab complex was determined by x-ray crystallography to 1.9 A resolution revealing a conformational epitope (876 A(2)) comprised of the planar surface of the four-stranded anti-parallel beta-sheet of Phl p 2. The IgE-defined dominant epitope is discontinuous and formed by 21 residues located mostly within the beta strands. Of the 21 residues, 9 interact directly with 5 of the 6 CDRs (L1, L3, H1, H2, H3) of the IgE Fab predominantly by hydrogen bonding and van der Waals interactions. Our results indicate that IgE Abs recognize conformational epitopes with high affinity and provide a structural basis for the highly efficient effector cell activation by allergen/IgE immune complexes.

High-affinity IgE recognition of a conformational epitope of the major respiratory allergen Phl p 2 as revealed by X-ray crystallography.,Padavattan S, Flicker S, Schirmer T, Madritsch C, Randow S, Reese G, Vieths S, Lupinek C, Ebner C, Valenta R, Markovic-Housley Z J Immunol. 2009 Feb 15;182(4):2141-51. PMID:19201867^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Padavattan S, Flicker S, Schirmer T, Madritsch C, Randow S, Reese G, Vieths S, Lupinek C, Ebner C, Valenta R, Markovic-Housley Z. High-affinity IgE recognition of a conformational epitope of the major respiratory allergen Phl p 2 as revealed by X-ray crystallography. J Immunol. 2009 Feb 15;182(4):2141-51. PMID:19201867 doi:182/4/2141

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vxq"

@@ Line 2: / Line 2: @@
 <StructureSection load='2vxq' size='340' side='right' caption='[[2vxq]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vxq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Phleum_pratense Phleum pratense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VXQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vxq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_timothy Common timothy] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VXQ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bmw|1bmw]], [[1whp|1whp]], [[1who|1who]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vxq RCSB], [http://www.ebi.ac.uk/pdbsum/2vxq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxq OCA], [http://pdbe.org/2vxq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vxq RCSB], [http://www.ebi.ac.uk/pdbsum/2vxq PDBsum]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 14: / Line 14: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vxq ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 24: / Line 24: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2vxq" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens]]
+[[Category: Common timothy]]
-[[Category: Phleum pratense]]
+[[Category: Human]]
 [[Category: Ebner, C]]
 [[Category: Flicker, S]]

2vxq

From Proteopedia

Revision as of 15:03, 7 February 2016

CRYSTAL STRUCTURE OF THE MAJOR GRASS POLLEN ALLERGEN PHL P 2 IN COMPLEX WITH ITS SPECIFIC IGE-FAB

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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