2o95
From Proteopedia
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|PDB= 2o95 |SIZE=350|CAPTION= <scene name='initialview01'>2o95</scene>, resolution 1.950Å | |PDB= 2o95 |SIZE=350|CAPTION= <scene name='initialview01'>2o95</scene>, resolution 1.950Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=ETE:2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>ETE</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PSMD7, Mov34L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= PSMD7, Mov34L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2o96|2O96]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o95 OCA], [http://www.ebi.ac.uk/pdbsum/2o95 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o95 RCSB]</span> | ||
}} | }} | ||
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[[Category: Sanches, M.]] | [[Category: Sanches, M.]] | ||
[[Category: Zanchin, N I.T.]] | [[Category: Zanchin, N I.T.]] | ||
| - | [[Category: 12P]] | ||
| - | [[Category: ETE]] | ||
| - | [[Category: PG4]] | ||
| - | [[Category: PGE]] | ||
| - | [[Category: SO4]] | ||
[[Category: 26s proteasome]] | [[Category: 26s proteasome]] | ||
[[Category: jab1/mpn]] | [[Category: jab1/mpn]] | ||
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[[Category: psmd7]] | [[Category: psmd7]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:14:29 2008'' |
Revision as of 01:14, 31 March 2008
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| , resolution 1.950Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Gene: | PSMD7, Mov34L (Homo sapiens) | ||||||
| Related: | 2O96
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Metal-Free Dimeric Human Mov34 MPN domain (residues 1-186)
Overview
The 26S proteasome is a large protein complex involved in protein degradation. We have shown previously that the PSMD7/Mov34 subunit of the human proteasome contains a proteolytically resistant MPN domain. MPN domain family members comprise subunits of the proteasome, COP9-signalosome and translation initiation factor 3 complexes. Here, the crystal structure of two C-terminally truncated proteins, MPN 1-186 and MPN 1-177, were solved to 1.96 and 3.0 A resolution, respectively. MPN 1-186 is formed by nine beta-strands surrounded by three alpha-helices plus a fourth alpha-helix at the C terminus. This final alpha-helix emerges from the domain core and folds along with a symmetrically related subunit, typical of a domain swap. The crystallographic dimer is consistent with size-exclusion chromatography and DLS analysis showing that MPN 1-186 is a dimer in solution. MPN 1-186 shows an overall architecture highly similar to the previously reported crystal structure of the Archaeal MPN domain AfJAMM of Archaeoglobus fulgidus. However, previous structural and biophysical analyses have shown that neither MPN 1-186 nor full-length human Mov34 bind metal, in opposition to the zinc-binding AfJAMM structures. The zinc ligand residues observed in AfJAMM are conserved in the yeast Rpn11 proteasome and Csn5 COP-signalosome subunits, which is consistent with the isopeptidase activity described for these proteins. The results presented here show that, although the MPN domain of Mov34 shows a typical metalloprotease fold, it is unable to coordinate a metal ion. This finding and amino acid sequence comparisons can explain why the MPN-containing proteins Mov34/PSMD7, RPN8, Csn6, Prp8p and the translation initiation factor 3 subunits f and h do not show catalytic isopeptidase activity, allowing us to propose the hypothesis that in these proteins the MPN domain has a primarily structural function.
About this Structure
2O95 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of the human Mov34 MPN domain reveals a metal-free dimer., Sanches M, Alves BS, Zanchin NI, Guimaraes BG, J Mol Biol. 2007 Jul 27;370(5):846-55. Epub 2007 May 10. PMID:17559875
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