2ob2
From Proteopedia
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|PDB= 2ob2 |SIZE=350|CAPTION= <scene name='initialview01'>2ob2</scene>, resolution 1.920Å | |PDB= 2ob2 |SIZE=350|CAPTION= <scene name='initialview01'>2ob2</scene>, resolution 1.920Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PPM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= PPM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2ob1|2OB1]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ob2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ob2 OCA], [http://www.ebi.ac.uk/pdbsum/2ob2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ob2 RCSB]</span> | ||
}} | }} | ||
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[[Category: Mueller, I B.]] | [[Category: Mueller, I B.]] | ||
[[Category: Mueller-Dieckmann, J.]] | [[Category: Mueller-Dieckmann, J.]] | ||
| - | [[Category: | + | [[Category: ppm1 without 1,8-ans (as 1rjd)]] |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:15:14 2008'' |
Revision as of 01:15, 31 March 2008
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| , resolution 1.920Å | |||||||
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| Ligands: | , , , | ||||||
| Gene: | PPM1 (Saccharomyces cerevisiae) | ||||||
| Related: | 2OB1
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ppm1 in the absence of 1,8-ANS (cf 1JD)
Overview
A technique is described whereby the addition of low concentrations (millimolar to micromolar) of the fluorescent dye 1,8-ANS to the protein solution prior to crystallization results in crystallization experiments in which protein crystals are strongly contrasted above background artifacts when exposed to low-intensity UV radiation. As 1,8-ANS does not covalently modify the protein sample, no further handling or purification steps are necessary. The system has been tested on a wide variety of protein samples and it has been shown that the addition of 1,8-ANS has no discernible effect on the crystallization frequencies or crystallization conditions of these proteins. As 1,8-ANS interacts with a wide variety of proteins, this is proposed to be a general solution for the automated classification of protein crystallization images and the detection of protein crystals. The results also demonstrate the expected discrimination between salt and protein crystals, as well as allowing the straightforward identification of small crystals that grow in precipitate or under a protein skin.
About this Structure
2OB2 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye., Groves MR, Muller IB, Kreplin X, Muller-Dieckmann J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):526-35. Epub 2007, Mar 16. PMID:17372358
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