2ob7
From Proteopedia
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|PDB= 2ob7 |SIZE=350|CAPTION= <scene name='initialview01'>2ob7</scene> | |PDB= 2ob7 |SIZE=350|CAPTION= <scene name='initialview01'>2ob7</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1pv6|1PV6]], [[1n34|1N34]], [[1q2b|1Q2B]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ob7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ob7 OCA], [http://www.ebi.ac.uk/pdbsum/2ob7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ob7 RCSB]</span> | ||
}} | }} | ||
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[[Category: tmrna]] | [[Category: tmrna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:15:18 2008'' |
Revision as of 01:15, 31 March 2008
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| Ligands: | , , , | ||||||
| Related: | 1PV6, 1N34, 1Q2B
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of tmRNA-(SmpB)2 complex as inferred from cryo-EM
Overview
A eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer-messenger RNA, small protein B, and ribosome protein S1. By means of cryo-electron microscopy, we obtained a map of the complex composed of a stalled ribosome and small protein B, which appears near the decoding center. This result suggests that, when lacking a codon, the A-site on the small subunit is a target for small protein B. To investigate the role of S1 played in trans-translation, we obtained a cryo-electron microscopic map, including a stalled ribosome, transfer-messenger RNA, and small protein Bs but in the absence of S1. In this complex, several connections between the 30 S subunit and transfer-messenger RNA that appear in the +S1 complex are no longer found. We propose the unifying concept of scaffolding for the roles of small protein B and S1 in binding of transfer-messenger RNA to the ribosome during trans-translation, and we infer a pathway of sequential binding events in the initial phase of trans-translation.
About this Structure
2OB7 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1., Gillet R, Kaur S, Li W, Hallier M, Felden B, Frank J, J Biol Chem. 2007 Mar 2;282(9):6356-63. Epub 2006 Dec 19. PMID:17179154
Page seeded by OCA on Mon Mar 31 04:15:18 2008
Categories: Protein complex | Thermus thermophilus | Felden, B. | Frank, J. | Gillet, R. | Li, W. | Smpb | Tmrna
