2olu

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|PDB= 2olu |SIZE=350|CAPTION= <scene name='initialview01'>2olu</scene>, resolution 2.900&Aring;
|PDB= 2olu |SIZE=350|CAPTION= <scene name='initialview01'>2olu</scene>, resolution 2.900&Aring;
|SITE=
|SITE=
-
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= pbp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
|GENE= pbp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
 +
|DOMAIN=
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|RELATEDENTRY=[[2olv|2OLV]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2olu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2olu OCA], [http://www.ebi.ac.uk/pdbsum/2olu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2olu RCSB]</span>
}}
}}
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[[Category: Lovering, A L.]]
[[Category: Lovering, A L.]]
[[Category: Strynadka, N C.]]
[[Category: Strynadka, N C.]]
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[[Category: EDO]]
 
[[Category: lysozyme fold]]
[[Category: lysozyme fold]]
[[Category: transpeptidase fold glycosyltransferase family 51]]
[[Category: transpeptidase fold glycosyltransferase family 51]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:01:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:19:48 2008''

Revision as of 01:19, 31 March 2008


PDB ID 2olu

Drag the structure with the mouse to rotate
, resolution 2.900Å
Ligands: ,
Gene: pbp2 (Staphylococcus aureus)
Related: 2OLV


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Apoenzyme


Overview

Peptidoglycan glycosyltransferases (GTs) catalyze the polymerization step of cell-wall biosynthesis, are membrane-bound, and are highly conserved across all bacteria. Long considered the "holy grail" of antibiotic research, they represent an essential and easily accessible drug target for antibiotic-resistant bacteria, including methicillin-resistant Staphylococcus aureus. We have determined the 2.8 angstrom structure of a bifunctional cell-wall cross-linking enzyme, including its transpeptidase and GT domains, both unliganded and complexed with the substrate analog moenomycin. The peptidoglycan GTs adopt a fold distinct from those of other GT classes. The structures give insight into critical features of the catalytic mechanism and key interactions required for enzyme inhibition.

About this Structure

2OLU is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Structural insight into the transglycosylation step of bacterial cell-wall biosynthesis., Lovering AL, de Castro LH, Lim D, Strynadka NC, Science. 2007 Mar 9;315(5817):1402-5. PMID:17347437

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