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3ash

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Revision as of 09:39, 11 August 2016

MamA D159K mutant 1

Structural highlights

3ash is a 2 chain structure with sequence from Magsa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3as4, 3as5, 3as8, 3asf, 3asd, 3asg
Gene:	mam22, mamA (MAGSA)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The magnetosome, a biomineralizing organelle within magnetotactic bacteria, allows their navigation along geomagnetic fields. Magnetosomes are membrane-bound compartments containing magnetic nanoparticles and organized into a chain within the cell, the assembly and biomineralization of magnetosomes are controlled by magnetosome-associated proteins. Here, we describe the crystal structures of the magnetosome-associated protein, MamA, from Magnetospirillum magneticum AMB-1 and Magnetospirillum gryphiswaldense MSR-1. MamA folds as a sequential tetra-trico-peptide repeat (TPR) protein with a unique hook-like shape. Analysis of the MamA structures indicates two distinct domains that can undergo conformational changes. Furthermore, structural analysis of seven crystal forms verified that the core of MamA is not affected by crystallization conditions and identified three protein-protein interaction sites, namely a concave site, a convex site, and a putative TPR repeat. Additionally, relying on transmission electron microscopy and size exclusion chromatography, we show that highly stable complexes form upon MamA homooligomerization. Disruption of the MamA putative TPR motif or N-terminal domain led to protein mislocalization in vivo and prevented MamA oligomerization in vitro. We, therefore, propose that MamA self-assembles through its putative TPR motif and its concave site to create a large homooligomeric scaffold which can interact with other magnetosome-associated proteins via the MamA convex site. We discuss the structural basis for TPR homooligomerization that allows the proper function of a prokaryotic organelle.

Self-recognition mechanism of MamA, a magnetosome-associated TPR-containing protein, promotes complex assembly.,Zeytuni N, Ozyamak E, Ben-Harush K, Davidov G, Levin M, Gat Y, Moyal T, Brik A, Komeili A, Zarivach R Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):E480-7. Epub 2011 Jul 22. PMID:21784982^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zeytuni N, Ozyamak E, Ben-Harush K, Davidov G, Levin M, Gat Y, Moyal T, Brik A, Komeili A, Zarivach R. Self-recognition mechanism of MamA, a magnetosome-associated TPR-containing protein, promotes complex assembly. Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):E480-7. Epub 2011 Jul 22. PMID:21784982 doi:10.1073/pnas.1103367108

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ash"

@@ Line 1: / Line 1: @@
 ==MamA D159K mutant 1==
 <StructureSection load='3ash' size='340' side='right' caption='[[3ash]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ash]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Magnetospirillum_magneticum_amb-1 Magnetospirillum magneticum amb-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ASH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ASH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ash]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Magsa Magsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ASH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ASH FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3as4|3as4]], [[3as5|3as5]], [[3as8|3as8]], [[3asf|3asf]], [[3asd|3asd]], [[3asg|3asg]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mam22, mamA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342108 Magnetospirillum magneticum AMB-1])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mam22, mamA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342108 MAGSA])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ash FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ash OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ash RCSB], [http://www.ebi.ac.uk/pdbsum/3ash PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ash FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ash OCA], [http://pdbe.org/3ash PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ash RCSB], [http://www.ebi.ac.uk/pdbsum/3ash PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ash ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3ash" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Magnetospirillum magneticum amb-1]]
+[[Category: Magsa]]
 [[Category: Davidov, G]]
 [[Category: Levin, M]]

3ash

From Proteopedia

Revision as of 09:39, 11 August 2016

MamA D159K mutant 1

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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