2opc
From Proteopedia
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|PDB= 2opc |SIZE=350|CAPTION= <scene name='initialview01'>2opc</scene>, resolution 1.43Å | |PDB= 2opc |SIZE=350|CAPTION= <scene name='initialview01'>2opc</scene>, resolution 1.43Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene> | + | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= AvrL567-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5261 Melampsora lini]) | |GENE= AvrL567-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5261 Melampsora lini]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2opc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2opc OCA], [http://www.ebi.ac.uk/pdbsum/2opc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2opc RCSB]</span> | ||
}} | }} | ||
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[[Category: Teh, T.]] | [[Category: Teh, T.]] | ||
[[Category: Wang, C I.]] | [[Category: Wang, C I.]] | ||
| - | [[Category: CO]] | ||
| - | [[Category: IMD]] | ||
[[Category: avrl567-a]] | [[Category: avrl567-a]] | ||
[[Category: cobalt]] | [[Category: cobalt]] | ||
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[[Category: single-wavelength anomalous dispersion (sad)]] | [[Category: single-wavelength anomalous dispersion (sad)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:08 2008'' |
Revision as of 01:21, 31 March 2008
| |||||||
| , resolution 1.43Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | AvrL567-A (Melampsora lini) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Melampsora lini avirulence protein, AvrL567-A
Overview
Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
About this Structure
2OPC is a Single protein structure of sequence from Melampsora lini. Full crystallographic information is available from OCA.
Reference
The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein., Guncar G, Wang CI, Forwood JK, Teh T, Catanzariti AM, Ellis JG, Dodds PN, Kobe B, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):209-13. Epub 2007 Feb 23. PMID:17329816
Page seeded by OCA on Mon Mar 31 04:21:08 2008
