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3aei

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Revision as of 14:17, 11 August 2016

Crystal structure of the prefoldin beta2 subunit from Thermococcus strain KS-1

Structural highlights

3aei is a 2 chain structure with sequence from Thek1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2zqm, 2zdi
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Prefoldin (PFD) is a hexameric chaperone that captures a protein substrate and transfers it to a group II chaperonin (CPN) to complete protein folding. We have studied the interaction between PFD and CPN using those from a hyperthermophilic archaeon, Thermococcus strain KS-1 (T. KS-1). In this study, we determined the crystal structure of the T. KS-1 PFDbeta2 subunit and characterized the interactions between T. KS-1 CPNs (CPNalpha and CPNbeta) and T. KS-1 PFDs (PFDalpha1-beta1 and PFDalpha2-beta2). As predicted from its amino acid sequence, the PFDbeta2 subunit conforms to a structure similar to those of the PFDbeta1 subunit and the Pyrococcus horikoshii OT3 PFDbeta subunit, with the exception of the tip of its coiled-coil domain, which is thought to be the CPN interaction site. The interactions between T. KS-1 CPNs and PFDs (CPNalpha and PFDalpha1-beta1; CPNalpha and PFDalpha2-beta2; CPNbeta and PFDalpha1-beta1; and CPNbeta and PFDalpha2-beta2) were analyzed using the Biacore T100 system at various temperatures ranging from 20 to 45 degrees C. The affinities between PFDs and CPNs increased with an increase in temperature. The thermodynamic parameters calculated from association constants showed that the interaction between PFD and CPN is entropy driven. Among the four combinations of PFD-CPN interactions, the entropy difference in binding between CPNbeta and PFDalpha2-beta2 was the largest, and affinity significantly increased at higher temperatures. Considering that expression of PFDalpha2-beta2 and CPNbeta subunit is induced upon heat shock, our results suggest that PFDalpha1-beta1 is a general PFD for T. KS-1 CPNs, whereas PFDalpha2-beta2 is specific for CPNbeta.

Thermodynamic characterization of the interaction between prefoldin and group II chaperonin.,Sahlan M, Zako T, Tai PT, Ohtaki A, Noguchi K, Maeda M, Miyatake H, Dohmae N, Yohda M J Mol Biol. 2010 Jun 18;399(4):628-36. Epub 2010 Apr 29. PMID:20434454^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sahlan M, Zako T, Tai PT, Ohtaki A, Noguchi K, Maeda M, Miyatake H, Dohmae N, Yohda M. Thermodynamic characterization of the interaction between prefoldin and group II chaperonin. J Mol Biol. 2010 Jun 18;399(4):628-36. Epub 2010 Apr 29. PMID:20434454 doi:10.1016/j.jmb.2010.04.046

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3aei"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the prefoldin beta2 subunit from Thermococcus strain KS-1==
 <StructureSection load='3aei' size='340' side='right' caption='[[3aei]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3aei]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_sp. Thermococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AEI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AEI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3aei]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thek1 Thek1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AEI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AEI FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zqm|2zqm]], [[2zdi|2zdi]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aei OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aei RCSB], [http://www.ebi.ac.uk/pdbsum/3aei PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aei OCA], [http://pdbe.org/3aei PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aei RCSB], [http://www.ebi.ac.uk/pdbsum/3aei PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aei ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3aei" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Thermococcus sp]]
+[[Category: Thek1]]
 [[Category: Miyatake, H]]
 [[Category: Noguchi, K]]

3aei

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Revision as of 14:17, 11 August 2016

Crystal structure of the prefoldin beta2 subunit from Thermococcus strain KS-1

Structural highlights

Publication Abstract from PubMed

References

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