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3zgq

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Revision as of 09:00, 11 August 2016

Crystal structure of human interferon-induced protein IFIT5

Structural highlights

3zgq is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IFIT5_HUMAN] Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs.^[1] ^[2]

Publication Abstract from PubMed

Interferon-induced proteins, including the largely uncharacterized interferon-induced tetratricopeptide repeat (IFIT) protein family, provide defenses against pathogens. Differing from expectations for tetratricopeptide repeat (TPR) proteins and from human IFIT1, IFIT2, and IFIT3, we show that human IFIT5 recognizes cellular RNA instead of protein partners. In vivo and in vitro, IFIT5 bound to endogenous 5'-phosphate-capped RNAs, including transfer RNAs. The crystal structure of IFIT5 revealed a convoluted intramolecular packing of eight TPRs as a fold that we name the TPR eddy. Additional, non-TPR structural elements contribute to an RNA binding cleft. Instead of general cytoplasmic distribution, IFIT5 concentrated in actin-rich protrusions from the apical cell surface colocalized with the RNA-binding retinoic acid-inducible gene-I (RIG-I). These findings establish compartmentalized cellular RNA binding activity as a mechanism for IFIT5 function and reveal the TPR eddy as a scaffold for RNA recognition.

tRNA Binding, Structure, and Localization of the Human Interferon-Induced Protein IFIT5.,Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 , Jan 11. PMID:23317505^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. tRNA Binding, Structure, and Localization of the Human Interferon-Induced Protein IFIT5. Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 , Jan 11. PMID:23317505 doi:http://dx.doi.org/10.1016/j.molcel.2012.12.015
↑ Abbas YM, Pichlmair A, Gorna MW, Superti-Furga G, Nagar B. Structural basis for viral 5'-PPP-RNA recognition by human IFIT proteins. Nature. 2013 Jan 13. doi: 10.1038/nature11783. PMID:23334420 doi:http://dx.doi.org/10.1038/nature11783
↑ Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. tRNA Binding, Structure, and Localization of the Human Interferon-Induced Protein IFIT5. Mol Cell. 2013 Feb 21;49(4):743-50. doi: 10.1016/j.molcel.2012.12.015. Epub 2013 , Jan 11. PMID:23317505 doi:http://dx.doi.org/10.1016/j.molcel.2012.12.015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zgq"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human interferon-induced protein IFIT5==
 <StructureSection load='3zgq' size='340' side='right' caption='[[3zgq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zgq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZGQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zgq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZGQ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zgq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zgq RCSB], [http://www.ebi.ac.uk/pdbsum/3zgq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zgq OCA], [http://pdbe.org/3zgq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zgq RCSB], [http://www.ebi.ac.uk/pdbsum/3zgq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zgq ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/IFIT5_HUMAN IFIT5_HUMAN]] Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Also recognizes and binds tRNAs.<ref>PMID:23317505</ref> <ref>PMID:23334420</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 14: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zgq" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens]]
+[[Category: Human]]
 [[Category: Alber, T]]
 [[Category: Collins, K]]

3zgq

From Proteopedia

Revision as of 09:00, 11 August 2016

Crystal structure of human interferon-induced protein IFIT5

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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