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4d3l
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1 in the orthorhombic form== |
| + | <StructureSection load='4d3l' size='340' side='right' caption='[[4d3l]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4d3l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D3L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D3L FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HHD:(3S)-3-HYDROXYHEPTANEDIOIC+ACID'>HHD</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v2x|4v2x]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d3l OCA], [http://pdbe.org/4d3l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d3l RCSB], [http://www.ebi.ac.uk/pdbsum/4d3l PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Microbial degradation of the plant cell wall is a fundamental biological process with considerable industrial importance. Hydrolysis of recalcitrant polysaccharides is orchestrated by a large repertoire of carbohydrate-active enzymes that display a modular architecture in which a catalytic domain is connected via linker sequences to one or more noncatalytic carbohydrate-binding modules (CBMs). CBMs direct the appended catalytic modules to their target substrates, thus potentiating catalysis. The genome of the most abundant ruminal cellulolytic bacterium, Ruminococcus flavefaciens strain FD-1, provides an opportunity to discover novel cellulosomal proteins involved in plant cell-wall deconstruction. It encodes a modular protein comprising a glycoside hydrolase family 9 catalytic module (GH9) linked to two unclassified tandemly repeated CBMs (termed CBM-Rf6A and CBM-Rf6B) and a C-terminal dockerin. The novel CBM-Rf6A from this protein has been crystallized and data were processed for the native and a selenomethionine derivative to 1.75 and 1.5 A resolution, respectively. The crystals belonged to orthorhombic and cubic space groups, respectively. The structure was solved by a single-wavelength anomalous dispersion experiment using the CCP4 program suite and SHELXC/D/E. | ||
| - | + | Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.,Venditto I, Goyal A, Thompson A, Ferreira LM, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):45-8. doi:, 10.1107/S2053230X14025576. Epub 2015 Jan 1. PMID:25615967<ref>PMID:25615967</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4d3l" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | [[Category: Ferreira, L | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Ferreira, L M.A]] | ||
| + | [[Category: Fontes, C M.G A]] | ||
[[Category: Goyal, A]] | [[Category: Goyal, A]] | ||
[[Category: Najmudin, S]] | [[Category: Najmudin, S]] | ||
| + | [[Category: Thompson, A]] | ||
[[Category: Venditto, I]] | [[Category: Venditto, I]] | ||
| - | [[Category: | + | [[Category: Carbohydrate binding module]] |
| + | [[Category: Cel9a]] | ||
| + | [[Category: Cellulosome]] | ||
| + | [[Category: Glycoside hydrolase family 9]] | ||
| + | [[Category: Ruminococcus flavefaciens fd-1]] | ||
| + | [[Category: Sugar binding protein]] | ||
Revision as of 17:07, 20 January 2016
SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1 in the orthorhombic form
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