4rip
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==BromoUracil substituted structure of intercalation-locked DNA tetraplex== |
| + | <StructureSection load='4rip' size='340' side='right' caption='[[4rip]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4rip]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RIP FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rim|4rim]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rip RCSB], [http://www.ebi.ac.uk/pdbsum/4rip PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson-Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A-A base pairs between adjacent G-G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H-1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures. | ||
| - | + | An intercalation-locked parallel-stranded DNA tetraplex.,Tripathi S, Zhang D, Paukstelis PJ Nucleic Acids Res. 2015 Jan 27. pii: gkv033. PMID:25628357<ref>PMID:25628357</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Paukstelis, P]] | [[Category: Paukstelis, P]] | ||
| - | [[Category: Tripathi, S | + | [[Category: Tripathi, S K]] |
[[Category: Zhang, D]] | [[Category: Zhang, D]] | ||
| + | [[Category: Bromouracil]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: Homoduplex]] | ||
| + | [[Category: Intercalation-locked dna tetraplex]] | ||
Revision as of 11:53, 12 February 2015
BromoUracil substituted structure of intercalation-locked DNA tetraplex
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