2pk9
From Proteopedia
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|PDB= 2pk9 |SIZE=350|CAPTION= <scene name='initialview01'>2pk9</scene>, resolution 2.906Å | |PDB= 2pk9 |SIZE=350|CAPTION= <scene name='initialview01'>2pk9</scene>, resolution 2.906Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC ACID'>MES</scene> | + | |LIGAND= <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Cyclin-dependent_kinase Cyclin-dependent kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.22 2.7.11.22] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclin-dependent_kinase Cyclin-dependent kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.22 2.7.11.22] </span> |
|GENE= PHO85, SSG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PHO80, AGS3, TUP7, VAC5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= PHO85, SSG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PHO80, AGS3, TUP7, VAC5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2pmi|2PMI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pk9 OCA], [http://www.ebi.ac.uk/pdbsum/2pk9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pk9 RCSB]</span> | ||
}} | }} | ||
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[[Category: Shea, E K.O.]] | [[Category: Shea, E K.O.]] | ||
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
| - | [[Category: MES]] | ||
[[Category: cyclin]] | [[Category: cyclin]] | ||
[[Category: cyclin-dependent kinase]] | [[Category: cyclin-dependent kinase]] | ||
[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| + | [[Category: signaling protein,transferase/cell cycle complex]] | ||
[[Category: transferase/ cell cycle complex]] | [[Category: transferase/ cell cycle complex]] | ||
| - | [[Category: transferase/cell cycle complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:37:06 2008'' |
Revision as of 01:37, 31 March 2008
| |||||||
| , resolution 2.906Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | PHO85, SSG3 (Saccharomyces cerevisiae), PHO80, AGS3, TUP7, VAC5 (Saccharomyces cerevisiae) | ||||||
| Activity: | Cyclin-dependent kinase, with EC number 2.7.11.22 | ||||||
| Related: | 2PMI
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway
Overview
The ability to sense and respond appropriately to environmental changes is a primary requirement of all living organisms. In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes involved in the regulation of phosphate acquisition from the ambient environment. A signal transduction pathway (the PHO pathway) mediates this response, with Pho85-Pho80 playing a vital role. Here we report the X-ray structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex functioning in transcriptional regulation in response to environmental changes. The structure revealed a specific salt link between a Pho85 arginine and a Pho80 aspartate that makes phosphorylation of the Pho85 activation loop dispensable and that maintains a Pho80 loop conformation for possible substrate recognition. It further showed two sites on the Pho80 cyclin for high-affinity binding of the transcription factor substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant to each other and the active site.
About this Structure
2PK9 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:18042456
Page seeded by OCA on Mon Mar 31 04:37:06 2008
