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3zj8

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Revision as of 14:19, 27 April 2017

Crystal structure of strictosidine glucosidase in complex with inhibitor-2

Structural highlights

3zj8 is a 2 chain structure with sequence from Devilpepper. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3zj6, 3zj7
Activity:	3-alpha-(S)-strictosidine beta-glucosidase, with EC number 3.2.1.105
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SG1_RAUSE] Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the therapy of heart disorders.^[1]

Publication Abstract from PubMed

Abstract Insight into the structure and inhibition mechanism of O-beta-d-glucosidases by deoxa-pyranosylamine type inhibitors is provided by X-ray analysis of complexes between raucaffricine and strictosidine glucosidases and N-(cyclohexylmethyl)-, N-(cyclohexyl)- and N-(bromobenzyl)-beta-d-gluco-1,5-deoxa-pyranosylamine. All inhibitors anchored exclusively in the catalytic active site by competition with appropriate enzyme substrates. Thus facilitated prospective elucidation of the binding networks with residues located at <3.9 A distance will enable the development of potent inhibitors suitable for the production of valuable alkaloid glucosides, raucaffricine and strictosidine, by means of synthesis in Rauvolfia serpentina cell suspension cultures.

Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours.,Xia L, Lin H, Staniek A, Panjikar S, Ruppert M, Hilgers P, Williardt J, Rajendran C, Wang M, Warzecha H, Jager V, Stockigt J J Enzyme Inhib Med Chem. 2014 Aug 20:1-7. PMID:25140865^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stockigt J. Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity. ACS Chem Biol. 2011 Oct 28. PMID:22004291 doi:10.1021/cb200267w
↑ Xia L, Lin H, Staniek A, Panjikar S, Ruppert M, Hilgers P, Williardt J, Rajendran C, Wang M, Warzecha H, Jager V, Stockigt J. Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours. J Enzyme Inhib Med Chem. 2014 Aug 20:1-7. PMID:25140865 doi:http://dx.doi.org/10.3109/14756366.2014.949252

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zj8"

@@ Line 1: / Line 1: @@
 ==Crystal structure of strictosidine glucosidase in complex with inhibitor-2==
 <StructureSection load='3zj8' size='340' side='right' caption='[[3zj8]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zj6|3zj6]], [[3zj7|3zj7]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-alpha-(S)-strictosidine_beta-glucosidase 3-alpha-(S)-strictosidine beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.105 3.2.1.105] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zj8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zj8 RCSB], [http://www.ebi.ac.uk/pdbsum/3zj8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zj8 OCA], [http://pdbe.org/3zj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zj8 RCSB], [http://www.ebi.ac.uk/pdbsum/3zj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zj8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zj8" style="background-color:#fffaf0;"></div>
 ==See Also==

3zj8

From Proteopedia

Revision as of 14:19, 27 April 2017

Crystal structure of strictosidine glucosidase in complex with inhibitor-2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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