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4k0o

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Revision as of 07:24, 23 December 2016

F17b-G lectin domain with bound GlcNAc(beta1-3)Gal

Structural highlights

4k0o is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
NonStd Res:
Related:	1o9v, 2bs7, 2bs8, 3ffo
Gene:	f17bG, F17G ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[F17BG_ECOLX] Essential fimbrial adhesion factor that mediates binding to N-acetylglucosamine-containing receptors in the host intestinal microvilli, leading to colonization of the intestinal tissue, and diarrhea or septicemia. Also confers adhesiveness to laminin and basement membranes.

Publication Abstract from PubMed

Fimbriae are long, proteinaceous adhesion organelles expressed on the bacterial envelope, evolutionarily adapted by Escherichia coli strains for the colonization of epithelial linings. Using glycan arrays of the Consortium for Functional Glycomics (CFG), the lectin domains were screened of the fimbrial adhesins F17G and FedF from enterotoxigenic E. coli (ETEC) and of the FimH adhesin from uropathogenic E. coli. This has led to the discovery of a more specific receptor for F17G, GlcNAcb1,3Gal. No significant differences emerged from the glycan binding profiles of the F17G lectin domains from five different E. coli strains. However, strain-dependent amino acid variations, predominantly towards the positively charged arginine, were indicated by sulfate binding in FedF and F17G crystal structures. For FedF, no significant binders could be observed on the CFG glycan array. Hence, a shotgun array was generated from microvilli scrapings of the distal jejunum of a 3-week old piglet about to be weaned. On this array, the blood group A type 1 hexasaccharide emerged as a receptor for the FedF lectin domain and remarkably also for F18-fimbriated E. coli. F17G was found to selectively recognize glycan species with a terminal GlcNAc, typifying intestinal mucins. In conclusion, F17G and FedF recognize long glycan sequences that could only be identified using the shotgun approach. Interestingly, ETEC strains display a large capacity to adapt their fimbrial adhesins to ecological niches via charge-driven interactions, congruent with binding to thick mucosal surfaces displaying an acidic gradient along the intestinal tract.

Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli.,Lonardi E, Moonens K, Buts L, de Boer AR, Olsson JD, Weiss MS, Fabre E, Guerardel Y, Deelder AM, Oscarson S, Wuhrer M, Bouckaert J Biology (Basel). 2013 Jul 1;2(3):894-917. doi: 10.3390/biology2030894. PMID:24833052^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lonardi E, Moonens K, Buts L, de Boer AR, Olsson JD, Weiss MS, Fabre E, Guerardel Y, Deelder AM, Oscarson S, Wuhrer M, Bouckaert J. Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli. Biology (Basel). 2013 Jul 1;2(3):894-917. doi: 10.3390/biology2030894. PMID:24833052 doi:http://dx.doi.org/10.3390/biology2030894

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4k0o"

@@ Line 1: / Line 1: @@
 ==F17b-G lectin domain with bound GlcNAc(beta1-3)Gal==
 <StructureSection load='4k0o' size='340' side='right' caption='[[4k0o]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1o9v|1o9v]], [[2bs7|2bs7]], [[2bs8|2bs8]], [[3ffo|3ffo]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">f17bG, F17G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k0o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k0o RCSB], [http://www.ebi.ac.uk/pdbsum/4k0o PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k0o OCA], [http://pdbe.org/4k0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k0o RCSB], [http://www.ebi.ac.uk/pdbsum/4k0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k0o ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4k0o" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Adhesin|Adhesin]]
 == References ==
 <references/>

4k0o

From Proteopedia

Revision as of 07:24, 23 December 2016

F17b-G lectin domain with bound GlcNAc(beta1-3)Gal

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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