1gz1
From Proteopedia
(New page: 200px<br /> <applet load="1gz1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gz1, resolution 1.90Å" /> '''MUTANT D416A OF THE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GZ1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]] with NAG and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZ1 OCA]]. | + | 1GZ1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]] with NAG and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]]. Structure known Active Site: NAG. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZ1 OCA]]. |
==Reference== | ==Reference== | ||
Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9 A., Varrot A, Frandsen TP, Driguez H, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2201-4. Epub 2002, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454501 12454501] | Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9 A., Varrot A, Frandsen TP, Driguez H, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2201-4. Epub 2002, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454501 12454501] | ||
| + | [[Category: Cellulose 1,4-beta-cellobiosidase]] | ||
[[Category: Humicola insolens]] | [[Category: Humicola insolens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: thiooligosaccharide]] | [[Category: thiooligosaccharide]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:56:44 2007'' |
Revision as of 08:52, 30 October 2007
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MUTANT D416A OF THE CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS IN COMPLEX WITH METHYL-CELLOBIOSYL-4-DEOXY-4-THIO-BETA-D-CELLOBIOSIDE
Overview
The enzymatic degradation of cellulose continues to be one of the most, important enzyme-catalysed reactions. Glycoside hydrolases from family, GH-6 hydrolyse cellulose with inversion of the configuration of the, anomeric carbon. Whilst the catalytic proton donor has been clearly, identified (Asp226 in Humicola insolens Cel6A), the identification and, even the existence of a potential Bronsted base remains unclear. Equally, controversial is the role of surface-loop flexibility. Here, the structure, of the D416A mutant of the H. insolens cellobiohydrolase Cel6A in complex, with a non-hydrolysable thiooligosaccharide methyl, cellobiosyl-4-thio-beta-cellobioside at 1.9 A resolution is presented., Substrate distortion in the -1 subsite, to a (2)S(0) skew-boat, conformation, is observed, ... [(full description)]
About this Structure
1GZ1 is a [Single protein] structure of sequence from [Humicola insolens] with NAG and NA as [ligands]. Active as [Cellulose 1,4-beta-cellobiosidase], with EC number [3.2.1.91]. Structure known Active Site: NAG. Full crystallographic information is available from [OCA].
Reference
Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9 A., Varrot A, Frandsen TP, Driguez H, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2201-4. Epub 2002, Nov 23. PMID:12454501
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