2q9c
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2q9c |SIZE=350|CAPTION= <scene name='initialview01'>2q9c</scene>, resolution 2.20Å | |PDB= 2q9c |SIZE=350|CAPTION= <scene name='initialview01'>2q9c</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ftsY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus]) | |GENE= ftsY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2q9a|2Q9A]], [[2q9b|2Q9B]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q9c OCA], [http://www.ebi.ac.uk/pdbsum/2q9c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q9c RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Reyes, C L.]] | [[Category: Reyes, C L.]] | ||
[[Category: Stroud, R M.]] | [[Category: Stroud, R M.]] | ||
| - | [[Category: GNP]] | ||
| - | [[Category: SO4]] | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
[[Category: cell division]] | [[Category: cell division]] | ||
| Line 41: | Line 42: | ||
[[Category: srp]] | [[Category: srp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:46:32 2008'' |
Revision as of 01:46, 31 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | ftsY (Thermus aquaticus) | ||||||
| Related: | 2Q9A, 2Q9B
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of FTSY:GMPPNP with MGCL Complex
Overview
The signal recognition particle (SRP) and its conjugate receptor (SR) mediate cotranslational targeting of a subclass of proteins destined for secretion to the endoplasmic reticulum membrane in eukaryotes or to the plasma membrane in prokaryotes. Conserved active site residues in the GTPase domains of both SRP and SR mediate discrete conformational changes during formation and dissociation of the SRP.SR complex. Here, we describe structures of the prokaryotic SR, FtsY, as an apo protein and in two different complexes with a non-hydrolysable GTP analog (GMPPNP). These structures reveal intermediate conformations of FtsY containing GMPPNP and explain how the conserved active site residues position the nucleotide into a non-catalytic conformation. The basis for the lower specificity of binding of nucleotide in FtsY prior to heterodimerization with the SRP conjugate Ffh is also shown. We propose that these structural changes represent discrete conformational states assumed by FtsY during targeting complex formation and dissociation.
About this Structure
2Q9C is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates., Reyes CL, Rutenber E, Walter P, Stroud RM, PLoS ONE. 2007 Jul 11;2(7):e607. PMID:17622352
Page seeded by OCA on Mon Mar 31 04:46:32 2008
Categories: Single protein | Thermus aquaticus | Reyes, C L. | Stroud, R M. | Cell cycle | Cell division | Ffh | Ftsy | Gdp | Gtp-binding | Gtpase | Inner membrane | Membrane | Nucleotide-binding | Ribonucleoprotein | Rna-binding | Signal recognition particle | Srp
