5aim

From Proteopedia

(Difference between revisions)

Revision as of 12:52, 24 June 2015

Crystal structure of T138 central eWH domain

Structural highlights

5aim is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	5aio
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[TFC3_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding.^[1] ^[2] ^[3]

Publication Abstract from PubMed

In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for transcribing genes encoding tRNAs and other short non-coding RNAs. The recruitment of Pol III to tRNA-encoding genes requires the transcription factors (TF) IIIB and IIIC. TFIIIC has been described as a conserved, multi-subunit protein complex composed of two subcomplexes, called tauA and tauB. How these two subcomplexes are linked and how their interaction affects the formation of the Pol III pre-initiation complex (PIC) is poorly understood. Here we use chemical crosslinking mass spectrometry and determine the molecular architecture of TFIIIC. We further report the crystal structure of the essential TPR array from tauA subunit tau131 and characterize its interaction with a central region of tauB subunit tau138. The identified tau131-tau138 interacting region is essential in vivo and overlaps with TFIIIB-binding sites, revealing a crucial interaction platform for the regulation of tRNA transcription initiation.

Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.,Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Muller CW Nat Commun. 2015 Jun 10;6:7387. doi: 10.1038/ncomms8387. PMID:26060179^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lefebvre O, Carles C, Conesa C, Swanson RN, Bouet F, Riva M, Sentenac A. TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10512-6. PMID:1279682
↑ Gabrielsen OS, Marzouki N, Ruet A, Sentenac A, Fromageot P. Two polypeptide chains in yeast transcription factor tau interact with DNA. J Biol Chem. 1989 May 5;264(13):7505-11. PMID:2651441
↑ Arrebola R, Manaud N, Rozenfeld S, Marsolier MC, Lefebvre O, Carles C, Thuriaux P, Conesa C, Sentenac A. Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding. Mol Cell Biol. 1998 Jan;18(1):1-9. PMID:9418847
↑ Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Muller CW. Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly. Nat Commun. 2015 Jun 10;6:7387. doi: 10.1038/ncomms8387. PMID:26060179 doi:http://dx.doi.org/10.1038/ncomms8387

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5aim"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of T138 central eWH domain==
+<StructureSection load='5aim' size='340' side='right' caption='[[5aim]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5aim]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AIM FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5aio|5aio]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aim OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5aim RCSB], [http://www.ebi.ac.uk/pdbsum/5aim PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/TFC3_YEAST TFC3_YEAST]] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. TFC3 is essential for cell viability. Cooperates with TFC6 in DNA binding.<ref>PMID:1279682</ref> <ref>PMID:2651441</ref> <ref>PMID:9418847</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for transcribing genes encoding tRNAs and other short non-coding RNAs. The recruitment of Pol III to tRNA-encoding genes requires the transcription factors (TF) IIIB and IIIC. TFIIIC has been described as a conserved, multi-subunit protein complex composed of two subcomplexes, called tauA and tauB. How these two subcomplexes are linked and how their interaction affects the formation of the Pol III pre-initiation complex (PIC) is poorly understood. Here we use chemical crosslinking mass spectrometry and determine the molecular architecture of TFIIIC. We further report the crystal structure of the essential TPR array from tauA subunit tau131 and characterize its interaction with a central region of tauB subunit tau138. The identified tau131-tau138 interacting region is essential in vivo and overlaps with TFIIIB-binding sites, revealing a crucial interaction platform for the regulation of tRNA transcription initiation.
-The entry 5aim is ON HOLD  until Paper Publication
+Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.,Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Muller CW Nat Commun. 2015 Jun 10;6:7387. doi: 10.1038/ncomms8387. PMID:26060179<ref>PMID:26060179</ref>
-Authors: Male, G., Glatt, S., Mueller, C.W.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Crystal structure of T138 central eWH domain
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
-[[Category: Mueller, C.W]]
+__TOC__
+</StructureSection>
+[[Category: Glatt, S]]
 [[Category: Male, G]]
-[[Category: Glatt, S]]
+[[Category: Mueller, C W]]
+[[Category: T138]]
+[[Category: Tfiiic]]
+[[Category: Transcription]]

5aim

From Proteopedia

Revision as of 12:52, 24 June 2015

Crystal structure of T138 central eWH domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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