User:Shai Biran/Practice Proteopedia tutorial shai4

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== Exploring the Structure ==
== Exploring the Structure ==
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The
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<scene name='Sandbox_4/Green_fp/1'>chromophore</scene>, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.<ref>PMID:8703075</ref>
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chromophore, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.<ref>PMID:8703075</ref>

Current revision

Contents

Bold and Interwiki

  1. In your assigned Sandbox page, now displaying your new text, click “edit this page”.
  2. We want to make the word “GFP” bold. Select the word “GFP" and click on the button in the toolbar above the text-editing area with a big “B”.
  3. The result should be three single quotation marks on either side of the word “GFP” like so: ’’’GFP’’’
  4. Now make “1ema” into an interwiki link, so that it leads to the Proteopedia page for the PDB entry 1ema. Do this by selecting the text “1ema” and clicking on the button in the toolbar above the text-editing area with a big, underlined, blue “Ab”.
  5. The result should be two square inward-facing brackets on either side of the word “1ema”. It should look like this: 1ema
  6. Save the page using the “Save page” button.

Your page should look like this: ↓↓↓↓↓↓↓↓↓↓↓↓

Your Heading Here (maybe something like 'Structure')

Caption for this structure

Drag the structure with the mouse to rotate

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References

  1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
  3. ↑ Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075

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Shai Biran

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