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2qcx
From Proteopedia
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|SITE= <scene name='pdbsite=AC1:Pf1+Binding+Site+For+Residue+A+300'>AC1</scene> and <scene name='pdbsite=AC2:Pf1+Binding+Site+For+Residue+B+300'>AC2</scene> | |SITE= <scene name='pdbsite=AC1:Pf1+Binding+Site+For+Residue+A+300'>AC1</scene> and <scene name='pdbsite=AC2:Pf1+Binding+Site+For+Residue+B+300'>AC2</scene> | ||
|LIGAND= <scene name='pdbligand=PF1:N-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-N-(2-HYDROXYETHYL)FORMAMIDE'>PF1</scene> | |LIGAND= <scene name='pdbligand=PF1:N-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-N-(2-HYDROXYETHYL)FORMAMIDE'>PF1</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Thiaminase Thiaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.2 3.5.99.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiaminase Thiaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.2 3.5.99.2] </span> |
|GENE= tenA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |GENE= tenA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qcx OCA], [http://www.ebi.ac.uk/pdbsum/2qcx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qcx RCSB]</span> | ||
}} | }} | ||
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[[Category: Jenkins, A L.]] | [[Category: Jenkins, A L.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
| - | [[Category: PF1]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
[[Category: up-down bundle]] | [[Category: up-down bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:47:49 2008'' |
Revision as of 01:47, 31 March 2008
| |||||||
| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | |||||||
| Gene: | tenA (Bacillus subtilis) | ||||||
| Activity: | Thiaminase, with EC number 3.5.99.2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Bacillus subtilis TenA Y112F mutant complexed with formyl aminomethyl pyrimidine
Overview
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.
About this Structure
2QCX is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Mutagenesis studies on TenA: A thiamin salvage enzyme from Bacillus subtilis., Jenkins AL, Zhang Y, Ealick SE, Begley TP, Bioorg Chem. 2008 Feb;36(1):29-32. Epub 2007 Dec 3. PMID:18054064
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