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3j8a

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Revision as of 20:06, 15 December 2016

Structure of the F-actin-tropomyosin complex

Structural highlights

3j8a is a 7 chain structure with sequence from [1] and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Publication Abstract from PubMed

Filamentous actin (F-actin) is the major protein of muscle thin filaments, and actin microfilaments are the main component of the eukaryotic cytoskeleton. Mutations in different actin isoforms lead to early-onset autosomal dominant non-syndromic hearing loss, familial thoracic aortic aneurysms and dissections, and multiple variations of myopathies. In striated muscle fibres, the binding of myosin motors to actin filaments is mainly regulated by tropomyosin and troponin. Tropomyosin also binds to F-actin in smooth muscle and in non-muscle cells and stabilizes and regulates the filaments there in the absence of troponin. Although crystal structures for monomeric actin (G-actin) are available, a high-resolution structure of F-actin is still missing, hampering our understanding of how disease-causing mutations affect the function of thin muscle filaments and microfilaments. Here we report the three-dimensional structure of F-actin at a resolution of 3.7 A in complex with tropomyosin at a resolution of 6.5 A, determined by electron cryomicroscopy. The structure reveals that the D-loop is ordered and acts as a central region for hydrophobic and electrostatic interactions that stabilize the F-actin filament. We clearly identify map density corresponding to ADP and Mg2+ and explain the possible effect of prominent disease-causing mutants. A comparison of F-actin with G-actin reveals the conformational changes during filament formation and identifies the D-loop as their key mediator. We also confirm that negatively charged tropomyosin interacts with a positively charged groove on F-actin. Comparison of the position of tropomyosin in F-actin-tropomyosin with its position in our previously determined F-actin-tropomyosin-myosin structure reveals a myosin-induced transition of tropomyosin. Our results allow us to understand the role of individual mutations in the genesis of actin- and tropomyosin-related diseases and will serve as a strong foundation for the targeted development of drugs.

Structure of the F-actin--tropomyosin complex.,von der Ecken J, Muller M, Lehman W, Manstein DJ, Penczek PA, Raunser S Nature. 2014 Dec 1. doi: 10.1038/nature14033. PMID:25470062^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ von der Ecken J, Muller M, Lehman W, Manstein DJ, Penczek PA, Raunser S. Structure of the F-actin--tropomyosin complex. Nature. 2014 Dec 1. doi: 10.1038/nature14033. PMID:25470062 doi:http://dx.doi.org/10.1038/nature14033

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j8a"

@@ Line 1: / Line 1: @@
 ==Structure of the F-actin-tropomyosin complex==
 <StructureSection load='3j8a' size='340' side='right' caption='[[3j8a]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j8a RCSB], [http://www.ebi.ac.uk/pdbsum/3j8a PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8a OCA], [http://pdbe.org/3j8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j8a RCSB], [http://www.ebi.ac.uk/pdbsum/3j8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8a ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3j8a" style="background-color:#fffaf0;"></div>
 ==See Also==
 *[[Actin|Actin]]
+*[[Tropomyosin|Tropomyosin]]
 == References ==
 <references/>

3j8a

From Proteopedia

Revision as of 20:06, 15 December 2016

Structure of the F-actin-tropomyosin complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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