2qsc
From Proteopedia
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|PDB= 2qsc |SIZE=350|CAPTION= <scene name='initialview01'>2qsc</scene>, resolution 2.800Å | |PDB= 2qsc |SIZE=350|CAPTION= <scene name='initialview01'>2qsc</scene>, resolution 2.800Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qsc OCA], [http://www.ebi.ac.uk/pdbsum/2qsc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qsc RCSB]</span> | ||
}} | }} | ||
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[[Category: Stanfield, R L.]] | [[Category: Stanfield, R L.]] | ||
[[Category: Wilson, I A.]] | [[Category: Wilson, I A.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: ZN]] | ||
[[Category: aid]] | [[Category: aid]] | ||
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
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[[Category: virion]] | [[Category: virion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:52:43 2008'' |
Revision as of 01:52, 31 March 2008
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| , resolution 2.800Å | |||||||
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide
Overview
F425-B4e8 (B4e8) is a monoclonal antibody isolated from a human immunodeficiency virus type 1 (HIV-1)-infected individual that recognizes the V3 variable loop on the gp120 subunit of the viral envelope spike. B4e8 neutralizes a subset of HIV-1 primary isolates from subtypes B, C and D, which places this antibody among the very few human anti-V3 antibodies with notable cross-neutralizing activity. Here, the crystal structure of the B4e8 Fab' fragment in complex with a 24-mer V3 peptide (RP142) at 2.8 A resolution is described. The complex structure reveals that the antibody recognizes a novel V3 loop conformation, featuring a five-residue alpha-turn around the conserved GPGRA apex of the beta-hairpin loop. In agreement with previous mutagenesis analyses, the Fab' interacts primarily with V3 through side-chain contacts with just two residues, Ile(P309) and Arg(P315), while the remaining contacts are to the main chain. The structure helps explain how B4e8 can tolerate a certain degree of sequence variation within V3 and, hence, is able to neutralize an appreciable number of different HIV-1 isolates.
About this Structure
2QSC is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:18068724
Page seeded by OCA on Mon Mar 31 04:52:43 2008
Categories: Homo sapiens | Protein complex | Bell, C H. | Schiefner, A. | Stanfield, R L. | Wilson, I A. | Aid | Apoptosis | Envelope protein | Fab-peptide complex | Fusion protein | Glycoprotein | Gp120 | Hiv-1 | Host-virus interaction | Immune system | Immunoglobulin fold | Membrane | Transmembrane | V3 loop | Viral immunoevasion | Virion
