Sandbox Reserved 431
From Proteopedia
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==Quiz Question 2== | ==Quiz Question 2== | ||
| - | <Structure load='4c9w' size='300' frame='true' align='right' caption=' | + | <Structure load='4c9w' size='300' frame='true' align='right' caption='Active Site' scene='outline' /> |
| - | <scene name='48/483888/Outline/1'> | + | <scene name='48/483888/Outline/1'>Ligand Close Up</scene> |
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-If this protein binds to the active site of MTH1 is it competive or non competitive? | -If this protein binds to the active site of MTH1 is it competive or non competitive? | ||
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-I think here it would be best to show a close up ball and stick of the active site, one with hydrophobic representation, another with charges and one just showing the molecules. | -I think here it would be best to show a close up ball and stick of the active site, one with hydrophobic representation, another with charges and one just showing the molecules. | ||
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==See Also== | ==See Also== | ||
*[[MTH1]] (7,8-dihydro-8-oxoguanine triphosphatase) | *[[MTH1]] (7,8-dihydro-8-oxoguanine triphosphatase) | ||
Revision as of 12:59, 12 March 2015
| This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439. |
Contents |
Format Help
***We may want to consider changing our pdb code to 4c9x rather than 4c9w. The only difference between them is which enantiomer of crizotinib MTH1 is binding to. 4c9w = R and 4c9x = S. The S enantiomer is the one that causes inhibition. --Matt
OurMolecule
Introduction
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This is a sample scene created with SAT to by Group, and another to make of the protein.
MTH1, also known as 7,8-dihydro-8-oxoguanine triphosphatase is an enzyme in the human body encodet by the NUDT1 gene.
MTH1 is an enzyme also know as 7,8-dihydro-8-oxoguanine triphosphatase that in the human organism is encoded by the NUDT1 gene. It is inhibited by (S)-Crizotinib which induced an increase in DNA single-strand breaks, activated DNA repair in human colon carcinoma cells, and effectively suppressed tumor growth in animal models.
Additional Features
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Quiz Question 1
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- This scene colors the protein from the N-terminus to the C terminus.
- This scene colors nonpolar residues grey, polar residues red, and ligands bound to the protein green.
Close up of binding interaction - To be made.
Potential Questions:
-Would this protein be expected to embed itself in the cell membrane? Why?
-TBD
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-The questions are expected to have multiple parts, yes?
Quiz Question 2
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-If this protein binds to the active site of MTH1 is it competive or non competitive?
- What effect on the functionality of MTH1 would this have?
-Judging by the active site of the complex, why does the (S) enantiomer exhibit more affinity for MTH1 then the (R) enantiomer?
- If this question is too difficult, could ask something else like what features of the active site attract the crizotinib inhibitor.
-I think here it would be best to show a close up ball and stick of the active site, one with hydrophobic representation, another with charges and one just showing the molecules.
See Also
- MTH1 (7,8-dihydro-8-oxoguanine triphosphatase)
Credits
Introduction - Megi Marina
Overall Structure - Cassandra Martin
Drug Binding Site - Ben Ryter
Additional Features - Colin Hannahan
Quiz Question 1 - Matt Tuttle
Quiz Question 2 - Eric Rice
References
[http://www.nature.com/nature/journal/v508/n7495/pdf/nature13194.pdf Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy - Huber] http://en.wikipedia.org/wiki/NUDT1 https://us.axonmedchem.com/product/2296/?SID=5c93d8ee559b459b8b412a1c15d92d8c&country_id=US
