Sandbox Reserved 433
From Proteopedia
(Difference between revisions)
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==Overall Structure== | ==Overall Structure== | ||
| - | -Monomeric | ||
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-Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine protein kinase | -Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine protein kinase | ||
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-2 isoforms: alpha and beta, that are 97% homologous in their catalytic domain, but diverse at the N and C terminus. | -2 isoforms: alpha and beta, that are 97% homologous in their catalytic domain, but diverse at the N and C terminus. | ||
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-Both isoforms have a conserved N-terminal serine reside (S21 for alpha and S9 for beta) | -Both isoforms have a conserved N-terminal serine reside (S21 for alpha and S9 for beta) | ||
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-Phosphorylation of the N-terminal serine residue plays an important role for further activity | -Phosphorylation of the N-terminal serine residue plays an important role for further activity | ||
| - | + | -Classified the PDB structures into three groups a | |
| - | -Classified the PDB structures into three groups | + | |
Mishra, Nibha et al. Structure based virtual screening of GSK-3beta: Importance of protein flexibility and induced fit, 2009. Bioorganic & Medicinal Chemistry Letters, 2009, Vol. 19 Iss. 19, pp. 5582-5585. Retreived from http://www.sciencedirect.com/science/article/pii/S0960894X09011780 | Mishra, Nibha et al. Structure based virtual screening of GSK-3beta: Importance of protein flexibility and induced fit, 2009. Bioorganic & Medicinal Chemistry Letters, 2009, Vol. 19 Iss. 19, pp. 5582-5585. Retreived from http://www.sciencedirect.com/science/article/pii/S0960894X09011780 | ||
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<scene name='48/483890/1q3d/1'>1q3d structure</scene> | <scene name='48/483890/1q3d/1'>1q3d structure</scene> | ||
==Binding Interactions== | ==Binding Interactions== | ||
| - | 1)Folding of protein | ||
| - | a) location and description of position of alpha and beta sheets (Show in separate color on green screen) | ||
| - | b) | ||
| - | 2)Substrate binding | ||
| - | a) “phosphate-binding” pocket: describe three crucial basic residues (Show in different color on green screen) | ||
| - | b) GSK-3 substrates and binding specifics- S/TxxxS/T (S=Serine, T=threonine, X= any amino acid) | ||
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| - | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217193/ | ||
| - | http://www.rcsb.org/pdb/explore/explore.do?pdbId=1Q3D | ||
==Additional Features== | ==Additional Features== | ||
| - | + | 1. Difference that can be observed from complexes of Staurosporine with GSK-3 beta and other protein kinases such as CDK2, Chk1, Lck and PKA | |
| + | 2. Difference that can be observed from GSK-3 beta complexes with Staurosporine and other inhibitors (AMP-PNP, indirubin-3'-monoxime) | ||
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==Quiz Question 1== | ==Quiz Question 1== | ||
Which isoform of GSK-3 would work best for ___ function in ___ pathway? | Which isoform of GSK-3 would work best for ___ function in ___ pathway? | ||
Revision as of 22:09, 12 March 2015
| This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439. |
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