2qxj
From Proteopedia
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|PDB= 2qxj |SIZE=350|CAPTION= <scene name='initialview01'>2qxj</scene>, resolution 2.10Å | |PDB= 2qxj |SIZE=350|CAPTION= <scene name='initialview01'>2qxj</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=K7J:N-(3-CARBOXYPROPANOYL)-L-ALANYL-L-ALANYL-N-[(1S,2R)-1-BENZYL-2-HYDROXYPROPYL]-L-PROLINAMIDE'>K7J</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Stratum_corneum_chymotryptic_enzyme Stratum corneum chymotryptic enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.117 3.4.21.117] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Stratum_corneum_chymotryptic_enzyme Stratum corneum chymotryptic enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.117 3.4.21.117] </span> |
|GENE= KLK7, PRSS6, SCCE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= KLK7, PRSS6, SCCE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2qxg|2QXG]], [[2qxh|2QXH]], [[2qxi|2QXI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qxj OCA], [http://www.ebi.ac.uk/pdbsum/2qxj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qxj RCSB]</span> | ||
}} | }} | ||
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[[Category: Magdolen, V.]] | [[Category: Magdolen, V.]] | ||
[[Category: Schechter, N M.]] | [[Category: Schechter, N M.]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: K7J]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
[[Category: catalytic triad their]] | [[Category: catalytic triad their]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:54:28 2008'' |
Revision as of 01:54, 31 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | KLK7, PRSS6, SCCE (Homo sapiens) | ||||||
| Activity: | Stratum corneum chymotryptic enzyme, with EC number 3.4.21.117 | ||||||
| Related: | 2QXG, 2QXH, 2QXI
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Human Kallikrein 7 in Complex with Suc-Ala-Ala-Pro-Phe-chloromethylketone and Copper
Overview
hK7 or human stratum corneum chymotryptic enzyme belongs to the human tissue kallikrein (hKs) serine proteinase family and is strongly expressed in the upper layers of the epidermis. It participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer. We have solved x-ray structures of recombinant active hK7 at medium and atomic resolution in the presence of the inhibitors succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone and Ala-Ala-Phe-chloromethyl ketone. The most distinguishing features of hK7 are the short 70-80 loop and the unique S1 pocket, which prefers P1 Tyr residues, as shown by kinetic data. Similar to several other kallikreins, the enzyme activity is inhibited by Zn(2+) and Cu(2+) at low micromolar concentrations. Biochemical analyses of the mutants H99A and H41F confirm that only the metal-binding site at His(99) close to the catalytic triad accounts for the noncompetitive Zn(2+) inhibition type. Additionally, hK7 exhibits large positively charged surface patches, representing putative exosites for prime side substrate recognition.
About this Structure
2QXJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Chymotryptic specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7., Debela M, Hess P, Magdolen V, Schechter NM, Steiner T, Huber R, Bode W, Goettig P, Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16086-91. Epub 2007 Oct 1. PMID:17909180
Page seeded by OCA on Mon Mar 31 04:54:28 2008
Categories: Homo sapiens | Single protein | Stratum corneum chymotryptic enzyme | Bode, W. | Debela, M. | Goettig, P. | Hess, P. | Magdolen, V. | Schechter, N M. | Alternative splicing | Catalytic triad their | Copper | Glycoprotein | Histidine ligand | Hydrolase | Protease | Secreted | Serine protease | Zymogen
