2qzt
From Proteopedia
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|PDB= 2qzt |SIZE=350|CAPTION= <scene name='initialview01'>2qzt</scene>, resolution 1.700Å | |PDB= 2qzt |SIZE=350|CAPTION= <scene name='initialview01'>2qzt</scene>, resolution 1.700Å | ||
|SITE= <scene name='pdbsite=AC1:Plm+Binding+Site+For+Residue+A+300'>AC1</scene>, <scene name='pdbsite=AC2:Plm+Binding+Site+For+Residue+B+301'>AC2</scene> and <scene name='pdbsite=AC3:Plm+Binding+Site+For+Residue+A+302'>AC3</scene> | |SITE= <scene name='pdbsite=AC1:Plm+Binding+Site+For+Residue+A+300'>AC1</scene>, <scene name='pdbsite=AC2:Plm+Binding+Site+For+Residue+B+301'>AC2</scene> and <scene name='pdbsite=AC3:Plm+Binding+Site+For+Residue+A+302'>AC3</scene> | ||
| - | |LIGAND= <scene name='pdbligand=PLM:PALMITIC ACID'>PLM</scene> | + | |LIGAND= <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Sterol Carrier Protein 2 Like 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7159 Aedes aegypti]) | |GENE= Sterol Carrier Protein 2 Like 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7159 Aedes aegypti]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1pz4|1PZ4]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qzt OCA], [http://www.ebi.ac.uk/pdbsum/2qzt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qzt RCSB]</span> | ||
}} | }} | ||
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[[Category: Forest, K T.]] | [[Category: Forest, K T.]] | ||
[[Category: Lan, Q.]] | [[Category: Lan, Q.]] | ||
| - | [[Category: PLM]] | ||
[[Category: cholesterol]] | [[Category: cholesterol]] | ||
[[Category: fatty acid]] | [[Category: fatty acid]] | ||
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[[Category: sterol carrier]] | [[Category: sterol carrier]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:55:01 2008'' |
Revision as of 01:55, 31 March 2008
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| , resolution 1.700Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | |||||||
| Gene: | Sterol Carrier Protein 2 Like 2 (Aedes aegypti) | ||||||
| Related: | 1PZ4
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Sterol Carrier Protein 2 Like 2 (SCP2-L2) from Aedes Aegypti
Overview
Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.
About this Structure
2QZT is a Single protein structure of sequence from Aedes aegypti. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure/function analysis of SCP-2-like2 reveals differences among SCP-2 family members., Dyer DH, Wessely V, Forest KT, Lan Q, J Lipid Res. 2008 Mar;49(3):644-53. Epub 2007 Dec 15. PMID:18084051
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