2r33
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2ot6|2OT6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r33 OCA], [http://www.ebi.ac.uk/pdbsum/2r33 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r33 RCSB]</span> | ||
}} | }} | ||
| Line 31: | Line 34: | ||
[[Category: vigna unguiculata]] | [[Category: vigna unguiculata]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:56:01 2008'' |
Revision as of 01:56, 31 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Related: | 2OT6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a Bowman-Birk inhibitor from Vigna unguiculata seeds
Overview
Different protease inhibitors including Bowman-Birk type (BBI) have been reported from the seeds of Vigna unguiculata. Protease isoinhibitors of double-headed Bowman-Birk type from the seeds of Vigna unguiculata have been purified and characterized. The BBI from Vigna unguiculata (Vu-BBI) has been found to undergo self-association to form very stable dimers and more complex oligomers, by size-exclusion chromatography and SDS-PAGE in the presence of urea. Many BBIs have been reported to undergo self-association to form homodimers or more complex oligomers in solution. Only one dimeric crystal structure of a BBI (pea-BBI) is reported to date. We report the three-dimensional structure of a Vu-BBI determined at 2.5 A resolution. Although, the inhibitor has a monomer fold similar to that found in other known structures of Bowman-Birk protease inhibitors, its quaternary structure is different from that commonly observed in this family. The structural elements responsible for the stability of monomer molecule and dimeric association are discussed. The Vu-BBI may use dimeric or higher quaternary association to maintain the physiological state and to execute its biological function.
About this Structure
2R33 is a Protein complex structure of sequences from Vigna unguiculata. This structure supersedes the now removed PDB entry 2OT6. Full crystallographic information is available from OCA.
Reference
Bowman-Birk protease inhibitor from the seeds of Vigna unguiculata forms a highly stable dimeric structure., Rao KN, Suresh CG, Biochim Biophys Acta. 2007 Oct;1774(10):1264-73. Epub 2007 Aug 3. PMID:17869196
Page seeded by OCA on Mon Mar 31 04:56:01 2008
