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3zlf

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Revision as of 17:22, 18 January 2017

Structure of group A Streptococcal enolase K312A mutant

Structural highlights

3zlf is a 4 chain structure with sequence from Strp6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3zlg, 3zlh
Activity:	Phosphopyruvate hydratase, with EC number 4.2.1.11
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ENO_STRP6] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. Binds plasminogen when expressed on the bacterial cell surface, potentially allowing the bacterium to acquire surface-associated proteolytic activity, which in turn contributes to tissue invasion and virulence.^[1]

Publication Abstract from PubMed

Group A Streptococcus (GAS) is a human pathogen that has the potential to cause invasive disease by binding and activating human plasmin(ogen). Streptococcal surface enolase (SEN) is an octameric alpha-enolase that is localized at the GAS cell surface. In addition to its glycolytic role inside the cell, SEN functions as a receptor for plasmin(ogen) on the bacterial surface, but the understanding of the molecular basis of plasmin(ogen) binding is limited. In this study, we determined the crystal and solution structures of GAS SEN and characterized the increased plasminogen binding by two SEN mutants. The plasminogen binding ability of SENK312A and SENK362A is ~2- and ~3.4-fold greater than for the wild-type protein. A combination of thermal stability assays, native mass spectrometry and X-ray crystallography approaches shows that increased plasminogen binding ability correlates with decreased stability of the octamer. We propose that decreased stability of the octameric structure facilitates the access of plasmin(ogen) to its binding sites, leading to more efficient plasmin(ogen) binding and activation.

Stability of the octameric structure affects plasminogen-binding capacity of streptococcal enolase.,Cork AJ, Ericsson DJ, Law RH, Casey LW, Valkov E, Bertozzi C, Stamp A, Jovcevski B, Aquilina JA, Whisstock JC, Walker MJ, Kobe B PLoS One. 2015 Mar 25;10(3):e0121764. doi: 10.1371/journal.pone.0121764., eCollection 2015. PMID:25807546^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pancholi V, Fischetti VA. alpha-enolase, a novel strong plasmin(ogen) binding protein on the surface of pathogenic streptococci. J Biol Chem. 1998 Jun 5;273(23):14503-15. PMID:9603964
↑ Cork AJ, Ericsson DJ, Law RH, Casey LW, Valkov E, Bertozzi C, Stamp A, Jovcevski B, Aquilina JA, Whisstock JC, Walker MJ, Kobe B. Stability of the octameric structure affects plasminogen-binding capacity of streptococcal enolase. PLoS One. 2015 Mar 25;10(3):e0121764. doi: 10.1371/journal.pone.0121764., eCollection 2015. PMID:25807546 doi:http://dx.doi.org/10.1371/journal.pone.0121764

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zlf"

@@ Line 1: / Line 1: @@
 ==Structure of group A Streptococcal enolase K312A mutant==
 <StructureSection load='3zlf' size='340' side='right' caption='[[3zlf]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zlg|3zlg]], [[3zlh|3zlh]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zlf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zlf RCSB], [http://www.ebi.ac.uk/pdbsum/3zlf PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zlf OCA], [http://pdbe.org/3zlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zlf RCSB], [http://www.ebi.ac.uk/pdbsum/3zlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zlf ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zlf" style="background-color:#fffaf0;"></div>
 ==See Also==

3zlf

From Proteopedia

Revision as of 17:22, 18 January 2017

Structure of group A Streptococcal enolase K312A mutant

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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