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4q5i

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Revision as of 17:29, 18 January 2017

Crystal structure of a T151A mutant of the E. coli FeoB G-domain

Structural highlights

4q5i is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3hyr, 3hyt, 4q00
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FEOB_ECOLI] GTP-driven Fe(2+) uptake system.^[1] ^[2]

Publication Abstract from PubMed

GDP release from GTPases is usually extremely slow and is in general assisted by external factors, such as association with guanine exchange factors or membrane-embedded GPCRs (G protein-coupled receptors), which accelerate the release of GDP by several orders of magnitude. Intrinsic factors can also play a significant role; a single amino acid substitution in one of the guanine nucleotide recognition motifs, G5, results in a drastically altered GDP release rate, indicating that the sequence composition of this motif plays an important role in spontaneous GDP release. In the present study, we used the GTPase domain from EcNFeoB (Escherichia coli FeoB) as a model and applied biochemical and structural approaches to evaluate the role of all the individual residues in the G5 loop. Our study confirms that several of the residues in the G5 motif have an important role in the intrinsic affinity and release of GDP. In particular, a T151A mutant (third residue of the G5 loop) leads to a reduced nucleotide affinity and provokes a drastically accelerated dissociation of GDP.

Exploring the correlation between the sequence composition of the nucleotide binding G5 loop of the FeoB GTPase domain (NFeoB) and intrinsic rate of GDP release.,Guilfoyle AP, Deshpande CN, Schenk G, Maher MJ, Jormakka M Biosci Rep. 2014 Dec 12;34(6):e00158. doi: 10.1042/BSR20140152. PMID:25374115^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kammler M, Schon C, Hantke K. Characterization of the ferrous iron uptake system of Escherichia coli. J Bacteriol. 1993 Oct;175(19):6212-9. PMID:8407793
↑ Marlovits TC, Haase W, Herrmann C, Aller SG, Unger VM. The membrane protein FeoB contains an intramolecular G protein essential for Fe(II) uptake in bacteria. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16243-8. Epub 2002 Nov 22. PMID:12446835 doi:10.1073/pnas.242338299
↑ Guilfoyle AP, Deshpande CN, Schenk G, Maher MJ, Jormakka M. Exploring the correlation between the sequence composition of the nucleotide binding G5 loop of the FeoB GTPase domain (NFeoB) and intrinsic rate of GDP release. Biosci Rep. 2014 Dec 12;34(6):e00158. doi: 10.1042/BSR20140152. PMID:25374115 doi:http://dx.doi.org/10.1042/BSR20140152

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4q5i"

@@ Line 1: / Line 1: @@
 ==Crystal structure of a T151A mutant of the E. coli FeoB G-domain==
 <StructureSection load='4q5i' size='340' side='right' caption='[[4q5i]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hyr|3hyr]], [[3hyt|3hyt]], [[4q00|4q00]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q5i RCSB], [http://www.ebi.ac.uk/pdbsum/4q5i PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q5i OCA], [http://pdbe.org/4q5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q5i RCSB], [http://www.ebi.ac.uk/pdbsum/4q5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q5i ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4q5i" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4q5i

From Proteopedia

Revision as of 17:29, 18 January 2017

Crystal structure of a T151A mutant of the E. coli FeoB G-domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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