Sandbox Reserved 1059
From Proteopedia
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NrdH is a redox protein part of a family of redox proteins. The other proteins that maintain the redox balance of this protein are three [http://en.wikipedia.org/wiki/Thioredoxin Thioredoxin] and three [http://en.wikipedia.org/wiki/Glutaredoxin glutaredoxin]-like proteins. Prokaryotes typically maintain redox homeostasis through low-molecular weight thiols (glutathione) and through proteins invovled in disulfide exchange (thioredoxins). | NrdH is a redox protein part of a family of redox proteins. The other proteins that maintain the redox balance of this protein are three [http://en.wikipedia.org/wiki/Thioredoxin Thioredoxin] and three [http://en.wikipedia.org/wiki/Glutaredoxin glutaredoxin]-like proteins. Prokaryotes typically maintain redox homeostasis through low-molecular weight thiols (glutathione) and through proteins invovled in disulfide exchange (thioredoxins). | ||
| - | The process of ribonucleotide reduction is one of the most fundamental biochemical process that is required for the existence of DNA-based life <ref name="Phulera">. | + | The process of ribonucleotide reduction is one of the most fundamental biochemical process that is required for the existence of DNA-based life <ref name="Phulera">...<ref>Phulera, S. and Mande, S. (2013). The crystal structure of ''Mycobacterium tuberculosis'' NrdH at 0.87 Angstrom suggests a possible mode of its activity. ''Biochemistry'' 53: 4056-4065. DOI 10.1021/bi400191z</ref>. It is the only de novo pathway to synthesize deoxyribonucleotides. Deoxyribonucleotides are the building blocks of DNA, and they are synthesized from ribonucleotides by reducing the 2’OH in a radical based reaction. The deoxyribonucleotides are then used as precursors for the process of DNA synthesis. This reaction is catalyzed by ribonucleotide reductases (RNRs). All RNRs are related. This is evident due to the catalytic core, which is structurally conserved across all extant RNRs. RNRs are essential for the processes of DNA replication and repair (DOI: REF). |
There are three classes of ribonucleotide reductases (RNRs). These classes are divided based on the mechanism of radical generation in the reaction. | There are three classes of ribonucleotide reductases (RNRs). These classes are divided based on the mechanism of radical generation in the reaction. | ||
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== Function == | == Function == | ||
| - | The main function is to act as a reducing partner of class 1B ribonucleotide reductase and for ribonucleotide reduction (RR), it is thought to supply electrons for this biochemical reaction. RR is one of the most fundamental biochemical processes that is required for DNA based life form to exist. Ribonucleotide reductases (RNRs) produce deoxyribonucleotides. These are precursors for DNA synthesis. <ref name="Phulera" | + | The main function is to act as a reducing partner of class 1B ribonucleotide reductase and for ribonucleotide reduction (RR), it is thought to supply electrons for this biochemical reaction. RR is one of the most fundamental biochemical processes that is required for DNA based life form to exist. Ribonucleotide reductases (RNRs) produce deoxyribonucleotides. These are precursors for DNA synthesis. <ref name="Phulera">. |
== Structure == | == Structure == | ||
Revision as of 20:22, 9 April 2015
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NrdH of Mycobacterium tuberculosis
NrdH is a redox protein part of a family of redox proteins. The other proteins that maintain the redox balance of this protein are three Thioredoxin and three glutaredoxin-like proteins. Prokaryotes typically maintain redox homeostasis through low-molecular weight thiols (glutathione) and through proteins invovled in disulfide exchange (thioredoxins).
The process of ribonucleotide reduction is one of the most fundamental biochemical process that is required for the existence of DNA-based life [1]. It is the only de novo pathway to synthesize deoxyribonucleotides. Deoxyribonucleotides are the building blocks of DNA, and they are synthesized from ribonucleotides by reducing the 2’OH in a radical based reaction. The deoxyribonucleotides are then used as precursors for the process of DNA synthesis. This reaction is catalyzed by ribonucleotide reductases (RNRs). All RNRs are related. This is evident due to the catalytic core, which is structurally conserved across all extant RNRs. RNRs are essential for the processes of DNA replication and repair (DOI: REF).
There are three classes of ribonucleotide reductases (RNRs). These classes are divided based on the mechanism of radical generation in the reaction.
Class I enzymes reduce nucleotide 5’-diphosphates, while the other 2 classes reduce ribonucleotide 5’-triphosphates [1]
