4xbn

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'''Unreleased structure'''
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==Crystal Structure of Human Galectin-3 CRD in Complex with Type 1 N-acetyllactosamine==
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<StructureSection load='4xbn' size='340' side='right' caption='[[4xbn]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4xbn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XBN FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xbl|4xbl]], [[4xbq|4xbq]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xbn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xbn RCSB], [http://www.ebi.ac.uk/pdbsum/4xbn PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN]] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Galectins represent beta-galactoside-binding proteins and are known to bind Galbeta1-3/4GlcNAc disaccharides (abbreviated as LN1 and LN2, respectively). Despite high sequence and structural homology shared by the carbohydrate recognition domain (CRD) of all galectin members, how each galectin displays different sugar-binding specificity still remains ambiguous. Herein we provided the first structural evidence of human galectins-1, 3-CRD and 7 in complex with LN1. Galectins-1 and 3 were shown to have higher affinity for LN2 than for LN1, while galectin-7 displayed the reversed specificity. In comparison with the previous LN2-complexed structures, the results indicated that the average glycosidic torsion angle of galectin-bound LN1 (psiLN1 approximately 135 degrees ) was significantly differed from that of galectin-bound LN2 (psiLN2 approximately -108 degrees ), i.e. the GlcNAc moiety adopted a different orientation to maintain essential interactions. Furthermore, we also identified an Arg-Asp/Glu-Glu-Arg salt-bridge network and the corresponding loop (to position the second Asp/Glu residue) critical for the LN1/2-binding preference.
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The entry 4xbn is ON HOLD until Paper Publication
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Structural Basis Underlying the Binding Preference of Human Galectins-1, -3 and -7 for Galbeta1-3/4GlcNAc.,Hsieh TJ, Lin HY, Tu Z, Huang BS, Wu SC, Lin CH PLoS One. 2015 May 6;10(5):e0125946. doi: 10.1371/journal.pone.0125946., eCollection 2015. PMID:25945972<ref>PMID:25945972</ref>
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Authors: Hsieh, T.J., Lin, H.Y., Lin, C.H.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Crystal Structure of Human Galectin-3 CRD in Complex with Type 1 N-acetyllactosamine
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Hsieh, T.J]]
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__TOC__
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[[Category: Lin, H.Y]]
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</StructureSection>
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[[Category: Lin, C.H]]
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[[Category: Hsieh, T J]]
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[[Category: Lin, C H]]
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[[Category: Lin, H Y]]
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[[Category: Complex]]
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[[Category: Human galectin-3 crd]]
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[[Category: Sugar binding protein]]
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[[Category: Type 1 lacnac]]

Revision as of 12:05, 20 May 2015

Crystal Structure of Human Galectin-3 CRD in Complex with Type 1 N-acetyllactosamine

4xbn, resolution 2.21Å

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